Identification of PM AP-associated proteins purified by NPA affinity chromatography
| Purified Protein | Post-translational Modifications | Internal Fragment Sequenced | GenBank Protein Identification No. | Amino Acids (Predicted) | Characterized Ortholog | Similarity |
|---|---|---|---|---|---|---|
| % | ||||||
| Aminopeptidases | ||||||
| FII-103 (AtAPM1) | GP (E,P) | (K)TLEVPTDLVALSN | CAB36783 | 879 | PS aminopeptidase M (mouse)/IRAP | 53 |
| FII-70 (AtAPP1) | GP (E,P) | (K)VSDEANSYFNGLG | CAB16823 | 634 | Aminopeptidase P1 (human) | 60 |
| FIII-42 (AtAPM1Δ) | GP (E,P) | (R)VATVVAHELAHQWF | CAB36783 | Unknown | Truncated form of AtAPM1 | ∼68 |
| AP-associated proteins | ||||||
| FIII-68 (AtMyA1) | GP (P) | (R)SVFASAALGK | AAB63637 | 619 | Myrosinase-associated protein (Brassica napus) | 62 |
| FII-55 (AtHSP70p) | GP (E,P) | (K)DVLK-(R)LVEHFAADEFNK | CAB10440 | 484 | HSP70 (Arabidopsis) | ∼68 |
| FII-44/66 (AtFAGP2) | GPI; GP (E,P) | (K)AFSDILKSTGADK | CAB40990 | 403 | Fasciclin-like arabinogalactan protein 2 (Arabidopsis) | 59 |
| FIII-40 (ATAPL1) | GP (E,P) | (K)LFVFGDSYADTGNI | CAB82926 | 359 | Anther-specific Pro-rich protein (Arabidopsis) | 46 |
| FIII-24 (AtGSTF2) | GP (P) | (K)NISQYAIMAIQ | P46422 | 212 | Auxin-binding GST II (Arabidopsis) | 100 |
Amino acid sequences of tryptic fragments from PM proteins contained in NPA affinity peaks II and III were obtained as described (see “Materials and Methods”). Protein identifications were assigned by NPA affinity fraction (FII or FIII) and SDS-PAGE apparent molecular mass. GenBank protein identification nos. were assigned if there was a unique database match with a similar predicted molecular mass. GP, Glycoprotein; GPI, glycosyl-phosphatidylinositol anchor; E, experimental evidence; P, predicted. K or R, Inferred lysine or arginine residues.