Specific [3H]NPA binding of PM vesicles, affinity fractions, and in vitro translation products of AtAPM1 and AtAPP1
| Sample | [3H]NPA Bound |
|---|---|
| pmol mg−1 | |
| PM | 0.1 ± 0.03 |
| Fraction I | 0.0 ± 0.03 |
| Fraction II | 7.1 ± 1.67 |
| Fraction III | 8.2 ± 1.25 |
| Fraction IV | 17.2 ± 3.07 |
| Fraction II+ III | 17.8 ± 2.52 |
| Fraction II + III +APAb | 5.9 ± 3.55∗ |
| Fraction IV +APAb | 16.8 ± 3.25 |
| In vitro AtAPM1 | 3.2 ± 1.07 |
| In vitro AtAPP1 | 1.9 ± 1.6 |
Specific activity is calculated as [3H]NPA binding − [3H]NPA binding in the presence of 1,000× cold NPA. Fractions I through IV, NPA affinity fractions I–IV; APAb, mixed polyclonal anti-AtAPM1 and AtAPP1 antibodies (see “Materials and Methods”); in vitro, in vitro translation products. Values reported are means and sd of three independent experiments. Student-Newman-Keuls ANOVA indicates that the values reported are different from a normal distribution (P < 0.001). Values with an asterisk differ from their control in pairwise Student's t tests (P < 0.01).