Table II.

Inhibition of maize acyl-CoA elongase activity by anti-GL8 IgG

EnzymePreimmuneAnti-GL8Inhibition
%
Stearoyl-CoA  elongase0.73 ± 0.05 (6)0.44 ± 0.08 (5)40
3-Ketoacyl-CoA  synthase0.15 ± 0.02 (6)0.14 ± 0.03 (6)6.7
3-Hydroxyacyl-CoA  dehydrase104 ± 6 (4)102 ± 4 (4)2.0
(E) 2,3 Enoyl-CoA  reductase107 ± 5 (4)103 ± 5 (4)4.0

All enzymatic activities were measured using linear standard conditions. The stearoyl-CoA elongase activity was expressed as nanomoles per milligram per hour, the synthase as nanomoles per milligram per 15 min, and the dehydrase and reductase as nanomoles per milligram per 30 min. IgG and microsomal proteins were mixed in a ratio of 2:1. The numbers of independent experiments performed are indicated in parentheses; in each experiment, assays were performed in triplicate.