Table I.

Efficiency of A. tumefaciens-mediated maize transformation 1-a

Experiment DateNo. of Embryos InfectedNo. of Bialaphos-Resistant Events RecoveredNo. of Callus Events Expressinggus GeneNo. of Events Regenerated to Plants/No. of Events AttemptedTransformation Efficiency
%
09/15/0021212 /29.5
11/22/0082212 /22.4
12/08/002000NA0.0
01/29/0127645 /522.2
02/05/0163212 /23.2
02/12/01121222 /21.7
02/19/011711NT5.9
02/26/0126101 /13.8
03/21/01246191517 /187.7
03/26/0158444 /46.9
04/02/0195111 /11.1
04/11/0176555 /56.6
04/27/0135111 /12.9
04/30/01122876 /66.6
05/22/011751182 /26.3
Total1184655150 /515.5

NA, Not applicable; NT, not tested.

    • F1-a  All experiments used 400 mg L−1 cysteine in cocultivation medium.

    • F1-b  Transformation efficiency = (no. of bialaphos-resistant events recovered/no. of embryos infected) × 100.