Table II.

Real-time PCR primers used for evaluation of steady-state mRNA levels of APX and actin 2 (ACT2)

Primer
Protein Name and Intracellular LocalizationmRNA GenBank Accession No.NameSequencePosition to Stop Codon
APX1, cytosolic X59600 Apx1A5′-GTCCATTCGGAACAATGAGGTTTGAC-3′−587 bp
Apx1B5′-GTGGGCACCAGATAAAGCGACAAT-3′−262 bp
APX2, cytosolic X98275 Apx2A5′-TGATGTGAAGACGAAGACAGGAGGAC-3′−610 bp
Apx2B5′-CCCATCCGACCAAACACATCTCTTA-3′−305 bp
APX3, microsomal X98003 Apx3A5′-CCCAAAATCACATACGCAGACCTGTA-3′−603 bp
X98276 Apx3B5′-AGTTGTCAAACTTCAGCGGCTCTTG-3′−306 bp
APX4, microsomal AF441713 Apx4A5′-CTACTAAATCCGGGGGAGCCAATG-3′−659 bp
Apx4B5′-CTCTGTTGCATCACTCCTTCCAAAAT-3′−337 bp
APX5, microsomal AF441714 Apx5A5′-AGCTAAACCGTCCACACAACAAAGGT-3′−653 bp
Apx5B5′-GTCCCAAAGTGTGACCTCCAGAGAGA-3′−354 bp
APX6, cytosolic AV555486 Apx6A5′-TGCAAAACGAAATAAGGAAAGTGGTG-3′−497 bp
AI995973 Apx6B5′-CACTCAGGGTTTCTGGAGGTAGCTTG-3′−255 bp
sAPX, chloroplastic stromal X98925 ApxSA5′-TGCTAATGCTGGTCTTGTGAATGCTT-3′−625 bp
ApxSB5′-CCACTACGTTCTGGCCTAGATCTTCC-3′−209 bp
tAPX, chloroplastic thylakoidal X98926 ApxTA5′-CAGAATGGGACTTGATGACAAGGAAA-3′−604 bp
ApxTB5′-ATGCAGCCACATCTTCAGCATACTTC-3′−306 bp
ACT2 U41998 Act2A5′-ACCTTGCTGGACGTGACCTTACTGAT-3′−592 bp
Act2B5′-GTTGTCTCGTGGATTCCAGCAGCTT-3′−295 bp
Act2C5′-ATTCAGATGCCCAGAAGTCTTGTTCC-3′−366 bp
Act2D5′-ACCACCGATCCAGACACTGTACTTCC-3′−99 bp