Table II.

Primer sets for PCR amplification

GeneForward PrimerReverse Primer
Semiquantitative PCR
AtNRT2.1 5′-GAA ACG CCG GAG TTG CCT CTG TCT CTG GG 5′-CAC CAT AGC CAC AAC GGC AGT TAC AAG GG
 AtNRT2.2 5′-GAG CCG ACC AAC ACA AAG AAA AAG TAC 5′-TCT GCT GCT CCT CCT TAC TAT ATT CGC C
 AtNRT2.3 5′-AGG ACT AGT GAA CGG CTG TGC AGG CGG G 5′-AGA GAG CGG CGC GAG ACA AAA GGT GCA A
 AtNRT2.4 5′-ATG GTA ATA GAA GCA CAC TCG AGA AGA A 5′-TAA GGA GCG GCG TGA GAT GAA AGG TAT G
 AtNRT2.5 5′-CTC TCC ACC GCT CCC GCC GTC TAT TTC ACC 5′-CCT CCC GCA CCA GTC ATT CCC GAT ACC ACC
 AtNRT2.6 5′-AAC CGC CTT CTC ACT TAT GCT TAC AGC TCC 5′-GAG AAC GGC GGG AAA CAA AAG GAG CAA CAC
 AtNRT2.7 5′-CGG TAT CTC TCA GCT CCT TAT GCC TCT C 5′-GAT GAC GAA GGC CCA CAA CAC ATT CCA C
 AtApt1 5′-TCC CAG AAT CGC TAA GAT TGC C 5′-CCT TTC CCT TAA GCT CTG
Real-time PCR
 AtNRT2.2 5′-GCT ATG CTT TCT CGG TAG ATG GTA G 5′-AAT GTC ATG TTT GGT GAG GTT AAG A
 AtNRT2.5 5′-CTC TGC TTT CGC CGT TCT CTT GTT C 5′-CGC TGC TAT AAT CCC TGC TGT CTG G
 AtNRT2.6 5′-TTG TTT CCC GCC GTT CTC TTG 5′-TCT GTC CAT TCC GCT CCA TAG
 AtNRT2.7 5′-CCT TCA TCC TCG TCC GTT TC 5′-AAT TCG GCT ATG GTG GAG TA