Primer sets for PCR amplification
| Gene | Forward Primer | Reverse Primer |
|---|---|---|
| Semiquantitative PCR | ||
| AtNRT2.1 | 5′-GAA ACG CCG GAG TTG CCT CTG TCT CTG GG | 5′-CAC CAT AGC CAC AAC GGC AGT TAC AAG GG |
| AtNRT2.2 | 5′-GAG CCG ACC AAC ACA AAG AAA AAG TAC | 5′-TCT GCT GCT CCT CCT TAC TAT ATT CGC C |
| AtNRT2.3 | 5′-AGG ACT AGT GAA CGG CTG TGC AGG CGG G | 5′-AGA GAG CGG CGC GAG ACA AAA GGT GCA A |
| AtNRT2.4 | 5′-ATG GTA ATA GAA GCA CAC TCG AGA AGA A | 5′-TAA GGA GCG GCG TGA GAT GAA AGG TAT G |
| AtNRT2.5 | 5′-CTC TCC ACC GCT CCC GCC GTC TAT TTC ACC | 5′-CCT CCC GCA CCA GTC ATT CCC GAT ACC ACC |
| AtNRT2.6 | 5′-AAC CGC CTT CTC ACT TAT GCT TAC AGC TCC | 5′-GAG AAC GGC GGG AAA CAA AAG GAG CAA CAC |
| AtNRT2.7 | 5′-CGG TAT CTC TCA GCT CCT TAT GCC TCT C | 5′-GAT GAC GAA GGC CCA CAA CAC ATT CCA C |
| AtApt1 | 5′-TCC CAG AAT CGC TAA GAT TGC C | 5′-CCT TTC CCT TAA GCT CTG |
| Real-time PCR | ||
| AtNRT2.2 | 5′-GCT ATG CTT TCT CGG TAG ATG GTA G | 5′-AAT GTC ATG TTT GGT GAG GTT AAG A |
| AtNRT2.5 | 5′-CTC TGC TTT CGC CGT TCT CTT GTT C | 5′-CGC TGC TAT AAT CCC TGC TGT CTG G |
| AtNRT2.6 | 5′-TTG TTT CCC GCC GTT CTC TTG | 5′-TCT GTC CAT TCC GCT CCA TAG |
| AtNRT2.7 | 5′-CCT TCA TCC TCG TCC GTT TC | 5′-AAT TCG GCT ATG GTG GAG TA |