Table I.

ABA levels in wild-type and gpa1 seeds

TreatmentABA
Wsgpa1-2
nmol g dry wt−1
Control3.26 ± 0.493.11 ± 0.88
6% (w/v) Glc2.90 ± 0.353.16 ± 0.35

Sterilized seeds were kept in darkness at 4°C for 2 d, moved to 3.0 mL of 1× Murashige and Skoog medium plus or minus 6% (w/v) Glc. After being cultured in light at 23°C for 24 h, the seeds and medium were lyophilized in the same tube used for incubation and the ABA contents were determined.