Table I.

Novel mlo alleles

AlleleMutant IDs1-aMutational Event at MloEffect on MLO ProteinProtein Domain AffectedBgh ResistanceMutagen
mlo-12 Do 4122C1396 → AF240 → LSecond intracellular loopPartialNitrosomethylurea
mlo-16 Do 2376G1917 → A1-b Not applicableNot applicableFullEthymethane sulfonate (EMS)
mlo-27 Do 20211-c G2004 → AG318 → EThird intracellular loopFullEMS
mlo-28 Do 4228C1224 → TT222→ ISecond intracellular loopPartialNaN3
mlo-29 This studyC2051 → TP334→ LThird intracellular loopFullNaN3
mlo-30 Do 2234, 2235A2242 → T1-d Δ 6 amino acidsC terminus (intracellular)FullEMS

Nucleotide nos. based on the genomic Mlo DNA sequence starting from the translational start site (ATG).

    • F1-a  According to Habekuss and Hentrich (1988).

    • F1-b  Nucleotide substitution in the conserved 3′ splice site of intron 9.

    • F1-c  Also Do 2002, 2003, 2004, 2005, 2014, 2015, 2016, and 2019.

    • F1-d  Nucleotide substitution in the conserved 3′ splice site of intron 11.