Table I.

Level of tryptamine in transgenic T0 and T1 tobacco plants

pT-CTSpT-CYTpT-ER
T0T1T0T1T0
Mean1-a 50.584 (258.966)99.359 (660.784)20.366 (159.244)34.019 (227.981)0.591 (4.016)
se 8.097 (39.154)12.255 (110.704)2.464 (25.103)4.949 (23.318)0.132 (0.766)
Minimum6.061 (61.92)32.757 (321.367)10.407 (61.881)14.953 (76.025)0.041 (0.759)
Maximum98.949 (570.124) 196.966 1-b (1,277.465)37.327 (346.991)62.604 (353.936)1.380 (10.701)
  • F1-a  Data represent statistical analysis of the fluorimetric quantitation of tryptamine synthesized in vivo in transgenic plants harboring cassettes for targeting TDC to different subcellular compartments. Levels of tryptamine are in microgram per milligram of TSP or microgram per gram fresh wt of leaf material (in parentheses).

  • F1-b  The highest amount of tryptamine detected in fully expanded leaves of a transgenic T1 plant expressing recombinant TDC in the chloroplast is underlined.