Table II.

Deduction and quantitative estimation of structural isomers comprising M5G, M6G, M7G, and M8G formed in experiments using digitonin- and Triton X-100-solubilized fenugreek GMGT

Compounds Surviving β-Mannosidase ActionDeduced Structure before β-Mannosidase TreatmentRelative Amount %
DigitoninTX-100
se
M5G
 MGMMMMGMMM20 ± 326 ± 2
 MGMMMMGMM80 ± 375 ± 2
M6G
 MGMMMMMGMMMM10 ± 35 ± 5
 MGMMMMMGMMM75 ± 483 ± 4
 MGMMMMMGMM16 ± 012 ± 2
M7G
 MGMMMMMMGMMMMM8 ± 43 ± 3
 MGMMMMMMGMMMM41 ± 244 ± 4
 MGMMMMMMGMMM43 ± 244 ± 3
 MGMMMMMMGMM10 ± 29 ± 1
M8G
 MGMMMMMMMGMMMMMM1 ± 1 32-a
 MGMMMMMMMGMMMMM20 ± 4202-a
 MGMMMMMMMGMMMM34 ± 3352-a
 MGMMMMMMMGMMM30 ± 0272-a
 MGMMMMMMMGMM16 ± 5152-a

M, Unsubstituted mannose residue; G, galactose-substituted mannose residue; TX-100, Triton X-100. Structures read from nonreducing to reducing end. Thus, Embedded Image .

    • F2-a Single determination; otherwise, means of at least two independent experiments.