Table II.

Effect of different thiol-containing compounds (Cys, dithiothreitol, and reduced glutathione) and the S-containing amino acid Met on respiratory characteristics of wt and transgenic (AS8) tobacco cells

TreatmentRespirationCyt CapacityAOX Capacity
wtAS8wtAS8wtAS8
nmol O2mg−1 dry wt h−1
Control468 ± 45439 ± 301,085 ± 160904 ± 8290 ± 2829 ± 4
Cys550 ± 6684 ± 839 ± 832 ± 6522 ± 6314 ± 3
Dithiothreitol482 ± 59386 ± 461,084 ± 123725 ± 60207 ± 3012 ± 4
Glutathione606 ± 64430 ± 341,276 ± 105823 ± 3124 ± 611 ± 6
Met462 ± 29425 ± 411,100 ± 58817 ± 3869 ± 1246 ± 20

Treatments were for 4 h and at a concentration of 1 mm. Data are the mean ± se from three independent experiments.