The effect of pharmacological compounds on the Cys-induced loss of cyt pathway capacity in transgenic (AS8) cells
Treatment | cyt Capacity |
---|---|
% of Control | |
Cys | 3 ± 1 |
Cys +endothall | 28 ± 8 |
Cys + ruthenium red | 22 ± 4 |
Cys +flavone | 19 ± 4 |
Cys + cycloheximide | 12 ± 2 |
Cys +LaCl | 2 ± 1 |
Compounds were added 15 min prior to Cys addition, and cyt capacity was determined after 4 h. Compounds were used at the following concentrations: Cys (1mm), endothall (100 μm), ruthenium red (100 μM), flavone (1 mm), cycloheximide (360 μm), and LaCl (1 mm). Data are the mean ± se from three to five independent experiments. The untreated (control) cells had a cyt capacity of 1,132 ± 62 nmol O2 mg−1 dry wt h−1 (mean ± se).