Table IV.

The effect of pharmacological compounds on the Cys-induced loss of cyt pathway capacity in transgenic (AS8) cells

Treatmentcyt Capacity
% of Control
Cys3 ± 1
Cys +endothall28 ± 8
Cys + ruthenium red22 ± 4
Cys +flavone19 ± 4
Cys + cycloheximide12 ± 2
Cys +LaCl2 ± 1

Compounds were added 15 min prior to Cys addition, and cyt capacity was determined after 4 h. Compounds were used at the following concentrations: Cys (1mm), endothall (100 μm), ruthenium red (100 μM), flavone (1 mm), cycloheximide (360 μm), and LaCl (1 mm). Data are the mean ± se from three to five independent experiments. The untreated (control) cells had a cyt capacity of 1,132 ± 62 nmol O2 mg−1 dry wt h−1 (mean ± se).