Table I.

Relative activity of crude extracts from petals of rose cv Golden Gate and cv Fragrant Cloud and of purified OOMT1 and OOMT2 gene products with selected substrates

Substrate Tested and Substitution PatternCrude ExtractsGene Products
cv GoldenGatecv Fragrant CloudOOMT1OOMT2
meta-Dihydroxy
 Orcinol100100100100
 Resorcinol (1,3-dihydroxybenzene)62944935
 Phloroglucinol (1,3,5-trihydroxybenzene)217360
 3,5-Dihydroxyanisole (1-methoxy,3,5-dihydroxybenzene)81808073
meta-Methoxy-hydroxy
 Orcinol monomethyl ether734780193
 3,5-Dimethoxyphenol (1,3-dimethoxy,5-hydroxybenzene)61695679
 3-Methoxyphenol (1-methoxy,3-hydroxybenzene)41612586
ortho-Dihydroxy
 Catechol (1,2-dihydroxybenzene)28611030
 Protocatechuic aldehyde194510
 Pyrogallol (1,2,3-trihydroxybenzene)2452714
 Caffeic acid3300
para-Hydroxy
 4-Methoxyphenol (1-methoxy,4-hydroxybenzene)130217
 Coumaric acid0000
ortho-Methoxy-hydroxy
 Guaiacol (1-methoxy,2-hydroxybenzene)989938145
O-Cresol (2-hydroxytoluene)35422755
 Eugenol2641316
 Ferulic acid0000

For both extracts and purified enzymes, activity with orcinol was set arbitrarily as 100%. All substrates were tested at a 1 mmconcentration.