Table IV.

ALDH assays of maize mitochondrial preparations

SubstrateSubstrate ConcentrationKy21rf2a-m8122
Glycolaldehyde20 μm 12 ± 0.13.2 ± 0.4
4.0 mm 16 ± 27.8 ± 0.5
Acetaldehyde18 μm 18 ± 0.16.8 ± 0.8
1.8 mm 17 ± 48.1 ± 0.1

Mitochondria were purified from etiolated maize seedlings of the inbred line Ky21 or homozygous for rf2a-m8122 and ALDH assays were conducted as described in “Materials and Methods.” The data represent the average from two parallel experiments; in each experiment, ALDH activity was measured three times for each substrate concentration. ALDH activity is expressed as μmol NADH min mg protein−1.