ALDH assays of maize mitochondrial preparations
| Substrate | Substrate Concentration | Ky21 | rf2a-m8122 |
|---|---|---|---|
| Glycolaldehyde | 20 μm | 12 ± 0.1 | 3.2 ± 0.4 |
| 4.0 mm | 16 ± 2 | 7.8 ± 0.5 | |
| Acetaldehyde | 18 μm | 18 ± 0.1 | 6.8 ± 0.8 |
| 1.8 mm | 17 ± 4 | 8.1 ± 0.1 |
Mitochondria were purified from etiolated maize seedlings of the inbred line Ky21 or homozygous for rf2a-m8122 and ALDH assays were conducted as described in “Materials and Methods.” The data represent the average from two parallel experiments; in each experiment, ALDH activity was measured three times for each substrate concentration. ALDH activity is expressed as μmol NADH min mg protein−1.