Table IV.

Compositions of total cell wall materials from endosperm tissues of T1 and T2 generation seeds from transgenic tobacco plants expressing fenugreek GMGT

Transgenic or Control LineCompositionMan/Gal Value4-b
AraGalGlcXyl4-aMan
mol %
T2generation
 FGT 117.017.04.461.73.5
16.115.14.464.34.1
 FGT 620.116.04.22.856.83.6
19.816.14.02.457.73.6
 FGT AA20.115.93.62.458.03.6
18.614.512.02.252.63.6
T1generation
 FGT 117.310.84.82.764.56.0
17.711.74.865.85.4
 FGT 317.311.55.465.85.5
17.611.75.365.45.4
 FGT 616.711.65.865.95.5
18.212.45.92.960.64.9
 FGT O16.911.95.465.85.3
18.814.35.061.94.2
 FGT L18.212.35.064.55.0
17.611.85.365.45.3
 FGT AA18.614.93.862.64.0
18.915.24.861.03.8
Controls
 CTR A18.410.23.567.96.4
19.510.04.02.464.26.4
 CTR B18.49.24.567.87.1
17.89.35.467.57.0
 CTR C19.010.23.82.464.56.3
17.710.23.768.36.4

The T1 generation seeds include 25% azygous individuals without the transgene, whereas the T2 generation seeds include less than 3% of such individuals. Transgenics were independent lines, and control plants had been regenerated from tissue culture. Duplicate analyses are of independent batches of 50 seeds.

    • F4-a Where not listed, included in Man total.

    • F4-b Where independent figure for Xyl not given, 2.6 mol % Xyl assumed