Table V.

Amounts and compositions of cell wall materials dissolved by the action of endo-(1→4)-β-d-mannanase on endosperm tissues of T1 and T2 generation seeds from transgenic tobacco plants expressing fenugreek GMGT

Composition5-a
Transgenic or Control LineAraGalGlcManMan/Gal Value% of Wall Dissolved% of Man Dissolved
mol %
T2generation
 FGT 13.310.36.679.87.75470
4.210.48.177.37.45169
 FGT 64.89.911.174.17.55878
5.010.19.076.07.56080
 FGT AA3.910.46.279.67.75675
4.310.76.178.87.45577
T1 generation
 FGT 14.47.06.981.711.762805-b
4.27.16.582.111.563855-b
 FGT 34.17.46.781.811.161805-b
4.07.87.181.110.463805-b
 FGT 64.07.56.981.510.958755-b
3.67.37.082.111.260805-b
 FGT O3.67.47.581.511.05875
4.37.27.281.211.25774
 FGT L3.78.37.180.89.75271
5.08.16.480.610.06279
 FGT AA4.28.36.081.59.85474
4.48.85.980.99.251705-b
Controls
 CTR A2.74.46.786.219.658805-b
2.84.25.687.420.86284
 CTR B3.34.15.986.821.26987
2.94.05.687.621.963855-b
 CTR C3.14.25.287.520.86182
3.74.55.886.119.16082

The T1 generation seeds include 25% azygous individuals without the transgene, whereas the T2 generation seeds include less than 3% of such individuals. Transgenics were independent lines, and control plants had been regenerated from tissue culture. Duplicate analyses are of independent lots of 50 endosperms.

    • F5-a Xyl absent from dissolved cell wall material.

    • F5-b Less precise value due to poor separation of Xyl from Man in hydrolysate of undissolved cell wall material.