Table I.

O2 consumption by isolated rice mitochondria following addition of different substrates, effectors, and inhibitors

Substrate−ADP+ADP+Myxothiazol
nmol O2 min−1 mg−1 protein
NADH87 ± 6199 ± 83 ± 1
Succinate99 ± 7198 ± 172 ± 1
Malate and pyruvate61 ± 9139 ± 111 ± 1
Cytochrome c438 ± 29
Membrane integrity96.1 ± 1%

Respiratory assays were performed according to “Materials and Methods.” O2 consumption rates are presented as means ± se (n > 3). Outer membrane integrity is presented as a percentage of intact mitochondria.