O2 consumption by isolated rice mitochondria following addition of different substrates, effectors, and inhibitors
Substrate | −ADP | +ADP | +Myxothiazol |
---|---|---|---|
nmol O2 min−1 mg−1 protein | |||
NADH | 87 ± 6 | 199 ± 8 | 3 ± 1 |
Succinate | 99 ± 7 | 198 ± 17 | 2 ± 1 |
Malate and pyruvate | 61 ± 9 | 139 ± 11 | 1 ± 1 |
Cytochrome c | 438 ± 29 | – | – |
Membrane integrity | 96.1 ± 1% | – | – |
Respiratory assays were performed according to “Materials and Methods.” O2 consumption rates are presented as means ± se (n > 3). Outer membrane integrity is presented as a percentage of intact mitochondria.