Table I.

rat mutants

*, Mutant complemented with wild-type gene; **, attempted complementation failed; ***, kanamycin (kan) resistance does not cosegregate with rat phenotype; +, mutant scores less than 25% of wild-type; ++, mutant scores less than 33% of wild-type; +++, mutant scores less than 50% of wild-type; ++++, mutant scores more than 50% of wild-type but still a rat mutant; +++++, mutant scores at the level of wild-type for transient GUS activity; N/A, not applicable.

Mutant Identificationa Collection Tumorigenesis Phosphinothricin (ppt) Resistance Transient GUS Zygosity Resistance Marker Gene Affected Insertion Site
rat1* F Feldmann + + + homo kan Arabinogalactan protein 5′-Untranslated region (UTR)
rat3* F Feldmann + + +++ homo kan Likely cell wall protein Intergenic
rat4* F Feldmann + + + homo kan Cellulose synthase-like protein (CslA-09) 3′-UTR
rat5* F Feldmann + + +++++ homo kan Histone H2A-1 3′-UTR
rat6 F Feldmann + + + kan
rat7 F Feldmann + + + kan Unknown protein
rat8 F Feldmann +++ +++ ++ kan
rat9 F Feldmann + + + kan Unknown protein
rat10 F Feldmann + + ++ kan
rat11 F Feldmann + + ++ kan
rat12 F Feldmann + + + kan
rat13 F Feldmann + + + kan
rat14 F Feldmann + + ++ kan Unknown protein 3′-UTR
rat15 F Feldmann + + + kan
rat16 F Feldmann + +++ ++ kan
rat17*** F Feldmann + + +++++ kan Myb transcription factor (cpc) 3′-UTR
rat18 F Feldmann + ++ +++++ kan
rat19 F Feldmann + + + kan Intergenic
rat20 F Feldmann + +++ +++++ kan
rat21 F Feldmann + + +++ kan
rat22 F Feldmann + ++ ++++ kan Unknown protein Intergenic
rat A1 F Feldmann + + homo kan
rat A2* F, R Feldmann + + + homo kan phosphatase 2A (rcn1) Sixth exon
rat A3 F Feldmann + + homo kan
rat A4 F Feldmann + ++++ homo kan Kinesin protein First intron
rat A5 F Feldmann + ++++ homo kan Unknown protein
rat A6 F Feldmann + + homo kan
ratJ1* F Feldmann + ++ + homo kan Importin β-3 18th intron
ratJ2 F Feldmann + + kan MADS box protein Fifth intron
ratJ3 F Feldmann + kan
ratJ4 F Feldmann + ++ kan
ratJ5 F Feldmann + + kan
ratJ6 F Feldmann + + kan 3- Isopropylmalate dehydrogenase Sixth exon
ratJ7 F Feldmann + + homo kan DEAD box RNA helicase Third intron
ratJ8 F Feldmann + + kan
ratJ9 F Feldmann + + kan Mitochondrial chaperonin hsp60 Fourth exon
ratJ10 F Feldmann ++ + kan
ratJ11 F Feldmann + + kan
ratJ12 F Feldmann + + kan
ratJ13 F Feldmann + + kan
ratJ14 F Feldmann + + kan
ratJR1 F Feldmann + + kan
ratJR2 F Feldmann ++ + kan
ratJR3 F Feldmann + + kan
ratJR4 F Feldmann ++ ++++ kan
ratJR5 F Feldmann ++ +++ kan
ratJR6 F Feldmann ++++ ++ kan
ratJR7 F Feldmann + + + kan
ratJR8 F Feldmann + ++ ++ kan
ratJR9 F Feldmann +++ + ++++ kan
ratJR10 F Feldmann ++++ + ++++ kan
ratJR11 F Feldmann ++++ + + kan
ratL1 F Bressan +++ ppt Cyclin/cinnamoyl transferase Third intron/3′-UTR
ratL2 F Bressan +++ ppt
ratL3 F Bressan +++ ppt ARID protein/METHF dehydrogenase 5′-UTR/Fourth exon
ratL4 F Bressan +++ ppt ATP citrate lyase/glucosidase 5′-UTR
ratL5 F Bressan + ppt A-T-rich repeat region Intergenic
ratL6 F Bressan ++ ppt Hypothetical protein Exon
ratL7 F Bressan + ppt CAAT repeat region 3′-UTR
ratT2 F Feldmann ++ ++ kan
ratT3 F Feldmann +++ + kan rac GTPase-activating protein 5′-UTR
ratT4 F Feldmann + + kan Ethylene-responsive element binding factor 3′-UTR
ratT5* F Feldmann + + ++ homo kan DREB2A Second exon
ratT6 F Feldmann + + kan
ratT7 F Feldmann + + kan
ratT8* F Feldmann + + + homo kan Receptor-like kinase 3′-UTR
ratT9 F Feldmann + + kan Receptor-like kinase 3′-UTR
ratT10 F Feldmann + + kan Unknown protein 3′-UTR
ratT11 F Feldmann + + kan
ratT12 F Feldmann + ++ kan
ratT13 F Feldmann + + kan Unknown protein 5′-UTR
ratT14 F Feldmann ++ +++ kan
ratT15 F Feldmann + + kan Unknown protein 5′-UTR
ratT16* F Feldmann + + + homo kan Unknown protein 6th intron
ratT17* F Feldmann + ++ ++++ homo kan Histone H3 Intergenic
ratT18 F Feldmann + + kan β-Expansin Intron
ratT19 F Feldmann +
uta1* F Bressan + homo ppt Voltage-dependent anion channel First exon
uta2 F Bressan + homo ppt F-box protein Exon
act2-1 R Feldmann + + ++ homo kan Actin-2 (root actin) First intron
act7-4* R Feldmann + + +++ homo kan Actin-7 (root actin) First intron
act7-1* R Feldmann + +++ +++ homo kan Actin-7 (root actin) Fourth intron
Importin α-7* R Feldmann + ++ +++ homo kan Importin α-7 Seventh intron
ratH1 R Feldmann + + +++ kan Unknown protein Intergenic
cep1 R Feldmann + + homo kan Constitutive expression of PR1,2,5 genes
atrx1* R Feldmann + homo kan Atrx1
rad5 R + ++++ ++ Point mutation
HTA2 R Feldmann ++ homo kan Histone H2A-2 5′-UTR
HTA3 R Feldmann + homo kan Histone H2A-3 3′-UTR
HTA10 R Feldmann ++++ +++++ homo kan Histone H2A-10 3′-UTR
HTA11 R Feldmann + homo kan Histone H2A-11 3′-UTR
HTA13 R Feldmann ++++ +++++ homo kan Histone H2A-13 5′-UTR
HTB5 R Feldmann ++ homo kan Histone H2B-5 5′-UTR
HTB6 R Feldmann ++ +++++ homo kan Histone H2B-6 3′-UTR
HTR4/5 F, R Feldmann +++ homo kan Histone H3-4/5 Intergenic
HFO3 R Feldmann + homo kan Histone H4-3 3′-UTR
HFO4 R Feldmann + +++++ homo kan Histone H4-4 3′-UTR
HAT6 R Feldmann + + +++ homo kan Histone acetyl transferase-6 5′-UTR
HAC11 R Feldmann ++ +++ homo kan Histone acetyl transferase-11 3′-UTR
HDA1 R Feldmann + +++++ homo kan Histone deacetylase-1 Exon
HDA2 R Feldmann ++++ +++++ homo kan Histone deacetylase-2 Exon
HDA6 R Feldmann +++ homo kan Histone deacetylase-6 3′-UTR
HDA9 R Feldmann ++++ homo kan Histone deacetylase-9 3′-UTR
HXA1 R Feldmann +++ + hetero kan Histone acetylase complex HXA1 5′-UTR
HXA2 R Feldmann + ++++ kan Component ADA2 homolog HXA2 5′-UTR
CS2491 R Feldmann + homo kan Disease resistance gene
Unknown (HAT4) R Feldmann + homo kan Homologous to phytoene hydroxylase
RNAi CHA 6A R ++++ hyg Chromatin-remodeling complex subunit 6 N/A
RNAi CHA 6C R ++++ hyg Chromatin- remodeling complex subunit 6 N/A
RNAi HAC8 R Line 156 A + homo hyg Histone acetyl transferase-8 N/A
R Line 156 B + homo hyg Histone acetyl transferase-8 N/A
RNAi NFA2 R Line 300 A +++ homo hyg Nucleosome assembly factor A N/A
R Line 422 A ++++ homo hyg Nucleosome assembly factor A N/A
RNAi SGA1 R Line 524 A ++++ homo hyg Chromatin-silencing group 1 N/A
R Line 524 B + homo hyg Chromatin-silencing group 1 N/A
RNAi BTI1 R Line 23 + + ppt Unknown protein N/A
RNAi BTI2 R Line 45 + + ppt Unknown protein N/A
RNAi BTI3 R Line 16 + + ppt Unknown protein N/A
RNAi AtRAB8 R Line 22 + + ppt AtRAB8 N/A
rat4 Antisense lines R Line L +++ hyg CsIA-09 N/A
R Line M ++++ hyg CsIA-09 N/A
R Line N ++++ hyg CsIA-09 N/A
Importin α-1 antisense lines R Line 1 +++ +++ ppt Importin α-1 N/A
R Line 2 ++++ ++++ ppt Importin α-1 N/A
R Line 3 ++ ++ ppt Importin α-1 N/A
R Line 4 +++ ++ ppt Importin α-1 N/A
BTI1 antisense R Line 132 ++ + + kan Unknown protein N/A
BTI2 antisense R Line 107 + +++ + kan Unknown protein N/A
RAB8 antisense R Line 19 + +++ +++ kan AtRAB8 N/A
VIP1 antisense R (In tobacco [Nicotiana tabacum]) + kan VirE2-interacting protein N/A
  • a Mutant identified by forward (F) or reverse (R) genetics