Table I.

Genes whose expression was altered 4 h after the withdrawal of P from the nutrient solution

Elements (P, K, or N) were withdrawn from the nutrient solutions supplied to 28-d-old Arabidopsis plants growing hydroponically. Gene expression profiles were determined in shoots 4 h after the withdrawal of P and 28 h after the withdrawal of K or N. Gene expression was calculated as the ratio of transcript abundance in shoots of plants starved of P, K, or N divided by transcript abundance in shoots of plants supplied with full nutrient solution.

Gene Expression Relative to Nutrient Replete Plants
GeneChip IDa Expression Levelb P Starved for 4 h K Starved for 28 h N Starved for 28 h Specificityc Other Stimulid Arabidopsis Genome Initiative (AGI) IDe Functional Categoryf
1 2 1 2 1 2
12097_g_at M 0.23 0.03 1.57 1.17 15.26 1.43 -P At2g20870 Expressed protein Unclassified
14633_at M 0.34 0.14 0.70 3.17 1.03 2.30 -P At5g54190 NADPH:protochlorophyllide oxidoreductase A Control of cellular organisation
15178_s_at L 0.23 0.18 0.52 0.75 0.71 1.01 -P t, hs, c, a, b At4g14130 Xyloglucan endotransglycosylase (XTR7) Metabolism
20518_at L 0.39 0.30 1.18 2.11 0.89 3.20 -P At1g10060 LEA76 homolog type 1 Metabolism
12115_at M 5.84 4.50 3.15 0.63 0.90 0.49 +P ms, c, cc At4g22470 Extensin-like protein Unclassified
12332_s_at H 18.10 4.27 0.88 0.20 5.30 0.37 +P At3g12500 Basic chitinase Cell rescue, defense, and virulence
12642_at M 2.54 2.90 1.64 0.71 2.96 0.72 +P At2g15390 Xyloglucan fucosyltransferase, putative Unclassified
12764_f_at H 2.80 4.20 0.32 1.04 2.38 0.84 +P cc At2g02930 Putative glutathione S-transferase Metabolism
13154_s_at H 4.48 11.29 2.07 0.13 2.35 0.09 +P cc At2g43590 Putative endochitinase Cell rescue, defense, and virulence
13244_s_at H 4.91 4.17 3.25 0.47 2.47 0.46 +P w At4g37990 Cinnamyl-alcohol dehydrogenase ELI3-2 Metabolism
13273_at H 3.17 6.96 2.99 1.11 2.42 0.90 +P hs, ms, cc At4g36990 Heat shock transcription factor HSF4 Transcription
13467_at H 2.94 2.74 1.08 0.53 1.08 0.76 +P At3g47420 Putative protein Transport facilitation
13842_at M 4.04 3.71 0.79 0.73 0.75 0.76 +P At1g24140 Putative metalloproteinase Protein fate
14016_s_at M 6.19 3.38 0.70 0.67 2.58 1.99 +P g, c At1g30700 FAD-linked oxidoreductase family Metabolism
14032_at M 2.59 3.47 1.78 0.43 3.59 0.58 +P lo, o, s, c At4g37370 Cytochrome P450-like protein Metabolism
14041_at L 3.45 2.59 0.95 0.79 2.14 0.90 +P At1g09480 Putative cinnamyl alcohol dehydrogenase Metabolism
14635_s_at M 3.16 7.84 0.83 1.23 2.22 0.72 +P c, d At2g14610 Pathogenesis-related PR-1-like protein Cell rescue, defense, and virulence
14636_s_at H 6.48 3.96 2.97 3.05 1.96 1.39 +PK w, c, d At1g75040 Thaumatin-like protein Cellular communication
14638_at H 2.99 2.57 2.13 0.42 2.76 0.35 +P Al, Oz, cc At3g49120 Putative peroxidase Cell rescue, defense, and virulence
14964_at H 2.91 6.73 1.03 0.45 0.61 0.20 +P At1g65500 Expressed protein Unclassified
15137_s_at H 2.86 5.70 0.57 0.30 0.89 0.37 +P cc At2g44790 Phytocyanin Unclassified
15162_at H 9.69 8.20 0.78 0.39 1.85 0.35 +P w, ms At3g04720 Hevein-like protein precursor (PR-4) Unclassified
15216_at H 2.99 2.89 1.44 1.47 1.38 0.98 +P cc At1g09560 Germin-like protein Metabolism
15415_at L 4.55 3.29 1.77 0.38 1.68 0.17 +P At1g22900 Disease resistance response protein-related Cell rescue, defense, and virulence
15665_at M 2.62 5.66 0.20 0.25 2.35 0.50 +P g, c, o At5g04340 Putative c2h2 zinc finger transcription factor Transcription
15672_s_at H 3.83 4.23 2.33 0.77 0.79 0.47 +P g, cc At2g22470 Arabinogalactan-protein (AGP2) Unclassified
15866_s_at H 2.99 3.36 1.76 0.95 2.50 0.85 +P At2g38860 Expressed protein Unclassified
15985_at M 4.03 3.53 0.43 0.61 0.65 0.37 +P Sulf At5g64100 Putative peroxidase Cell rescue, defense, and virulence
16014_at H 2.89 3.28 0.87 0.43 0.60 0.40 +P g, cc At1g75750 Expressed protein Unclassified
16053_i_at H 4.10 4.63 2.23 0.51 3.29 0.34 +P g, lo, w At1g02920 Glutathione S-transferase Metabolism
16150_at H 5.56 9.53 5.84 0.32 11.13 0.19 +P Al, c At4g12480 pEARLI 1 Unclassified
16198_at M 2.59 3.93 0.63 0.46 1.55 0.36 +P At2g28710 Putative C2H2-type zinc finger protein Transcription
16888_at M 4.64 4.12 2.24 0.31 2.07 0.30 +P At2g37770 Aldo/keto reductase family Metabolism
16914_s_at H 19.47 4.44 0.57 0.28 4.14 0.49 +P lo At4g11650 Osmotin precursor Cell rescue, defense, and virulence
17014_s_at H 3.92 59.55 3.87 0.66 0.57 0.22 +P P, d At2g02990 Ribonuclease, RNS1 Metabolism
17413_s_at H 14.85 43.39 0.12 0.48 5.73 0.18 +P lo At5g64120 Putative peroxidase Cell rescue, defense, and virulence
17485_s_at H 44.30 4.66 1.09 0.17 6.02 0.20 +P ms, cc At4g16260 Glycosyl hydrolase family 17 Metabolism
17533_s_at M 4.62 3.69 5.08 0.19 4.26 0.15 +P t, hs, c, a, b At4g25810 Xyloglucan endotransglycosylase (XTR-6) Metabolism
17840_at H 2.94 5.31 0.86 0.67 2.41 0.28 +P cc At2g43570 Endochitinase isolog Cell rescue, defense, and virulence
17894_at H 2.53 5.96 2.28 0.58 1.84 0.40 +P w At2g18690 Expressed protein Unclassified
17899_at H 4.69 11.87 1.68 0.21 1.35 0.23 +P o At4g15610 Expressed protein Unclassified
17907_s_at M 5.13 3.55 0.68 0.34 0.32 0.36 +P lo At2g37750 Expressed protein Unclassified
17930_s_at H 9.13 2.77 0.92 0.41 4.49 0.46 +P ms, lo At4g37520 Peroxidase, prxr2 Cell rescue, defense, and virulence
17963_at H 2.52 25.79 1.06 0.49 0.79 0.27 +P c At4g12470 pEARLI 1-like protein Unclassified
18217_g_at H 3.16 6.07 1.30 0.65 2.50 0.68 +P s, c At1g27730 Salt-tolerance zinc finger protein Transcription
18228_at H 5.83 13.73 3.40 0.65 4.14 0.59 +P ms At3g15356 Lectin-like protein Unclassified
18591_at M 2.83 3.32 2.16 0.73 2.22 0.38 +P o At5g08790 Expressed protein Unclassified
18888_at M 3.56 2.55 0.33 0.51 1.12 0.85 +P At1g15380 Expressed protein Unclassified
18966_at M 2.56 4.93 0.72 0.57 1.25 0.61 +P cc At2g29420 Glutathione transferase, putative Metabolism
19171_at H 4.46 7.62 2.89 0.34 2.48 0.31 +P ms, cc At2g43510 Putative trypsin inhibitor Unclassified
19178_at H 3.32 15.50 1.00 0.46 1.07 0.35 +P hl, c, o At5g20230 Blue copper-binding protein Unclassified
19284_at M 6.02 4.08 1.63 0.77 2.24 0.56 +P ms At2g38240 Putative anthocyanidin synthase Metabolism
19640_at H 2.54 11.96 3.39 0.54 3.39 0.29 +P ms At2g29460 Putative glutathione S-transferase Metabolism
19840_s_at M 3.54 6.47 3.72 0.67 1.93 0.29 +P cc At1g30720 FAD-linked oxidoreductase family Metabolism
19892_at H 2.84 5.76 2.19 0.64 1.40 0.36 +P cc At2g38870 Putative protease inhibitor Protein fate
19991_at H 2.51 7.87 0.50 0.58 1.77 0.47 +P g At2g35980 Similar to harpin-induced protein hin1 from tobacco Cell rescue, defense, and virulence
20194_at H 2.94 3.95 6.40 0.53 2.85 0.57 +P lo, cc At2g17500 Expressed protein Unclassified
20238_at H 3.09 4.09 2.49 1.10 1.95 0.37 +P At3g13790 Glycosyl hydrolase family 32 Metabolism
20269_at M 4.33 4.13 1.19 0.56 2.34 0.52 +P ms At2g45220 Pectinesterase family Metabolism
20287_at M 3.74 6.65 2.12 0.66 1.34 0.53 +P w At3g54420 Glycosyl hydrolase family 19 (class IV chitinase) Cell rescue, defense, and virulence
20420_at M 4.63 3.12 1.52 0.14 1.13 0.11 +P g,c At4g19810 Glycosyl hydrolase family 18/putative chitinase Cell rescue, defense, and virulence
20491_at H 8.86 4.00 2.85 0.27 2.22 0.28 +P w At2g29350 Putative tropinone reductase Unclassified
20499_at M 2.96 4.81 2.09 2.60 1.94 2.12 +P At2g33480 Putative NAM (no apical meristem)-like protein Development
20685_at M 2.55 4.72 2.00 0.85 1.18 0.36 +P o At4g13180 Short-chain alcohol dehydrogenase like protein Metabolism
  • a Affymetrix probe set number. b Qualitative estimates of absolute gene expression, classified as low (L, Affymetrix signal values < 150), medium (M) and high (H, Affymetrix signal values > 800). c Treatments for which genes were differentially expressed 2.5-fold in two biological replicates. (+), Genes whose expression has increased; (-), Genes whose expression has decreased. d Genes whose expression responds to auxin (a); aluminum (Al); brassinosteroids (b); cold (c); cell-cycle regulated (cc); drought (d); gravity (g); high light (hl); heat shock (hs); low oxygen (lo); mechanical stimulus (ms); oxidative stress (o); ozone (Oz); phosphate stress (P); salinity (s); sulfur deficiency (sulf); touch (t); and wounding and/or pathogen attack (w); see text for references. e AGI numbers and brief descriptions of the transcript identified by BLAST searching and from the descriptions provided by Ghassemian et al. (2002). f Functional categories for genes were identified using the AGI number to search the MIPS database (http://mips.gsf.de/proj/thal/db/; 28/11/02), where no category had been experimentally determined the category with the highest probability score was used.