The occurrence of novel cis-regulatory elements in the promoters of genes whose expression changed early or late in response to P withdrawal in the experiments reported here (Tables I and II) and cis-regulatory elements previously identified as common to genes whose expression responded to P starvation
The occurrence of these cis regulatory elements in the promoters of all genes on the Affymetrix GeneChip are given for comparison. —, not determined.
| No. of Genes with Element Upstream of Start Codon | ||||||||
|---|---|---|---|---|---|---|---|---|
| Element Name | Element Sequence | Early P-Responsive Genes | Late P-Responsive Genes | Affymetrix GeneChip | Reference | |||
| No. | % | No. | % | No. | % | |||
| Novel elements | ||||||||
| PHO-like | C(G/T/A)(C/T/A)GTGG | 18 | 32.1 | — | — | 588 | 7.1 | — |
| TATA box-like | TATAAATA | 20 | 35.7 | — | — | 753 | 9.1 | — |
| P-responsive elements | ||||||||
| PHR1-binding site | GNATATNC | 10 | 16.7 | 9 | 17.6 | 1498 | 18.2 | Rubio et al. (2001) |
| PHO element | CACGT(G/C) | 17 | 28.3 | 16 | 31.4 | 1574 | 19.1 | Mukatira et al. (2001) |
| ATGCCAT | 1 | 1.7 | 2 | 3.9 | 349 | 4.2 | Mukatira et al. (2001) | |
| Helix-loop-helix | CA(T/G)(A/C)TG | 32 | 53.3 | 23 | 45.1 | 4431 | 53.7 | Mukatira et al. (2001) |
| NIT 2 | TATC(A/T)(A/T) | 42 | 70.0 | 36 | 70.6 | 6296 | 76.3 | Mukatira et al. (2001) |