Table II.

Analysis of polar leaf lipids by HPLC a

Lipid Class Wild Type (n = 3)b LMIA-25 (n = 3)
MGDG 42.3 ± 0.4 41.0 ± 0.5
DGDG 12.6 ± 0.2 12.5 ± 0.8
PG 12.3 ± 0.3 12.8 ± 0.8
Phosphatidylethanolamine 11.6 ± 0.1 11.4 ± 0.2
Phosphatidylcholine 18.5 ± 0.3 19.2 ± 0.5
Sulfaquinovosyldiacylglycerol 2.5 ± 0.1 3.1 ± 0.3
Lipid content (μg mg-1 fresh leaf tissue) 2.9 ± 0.1 1.7 ± 0.3
Total chlorophyll (μg mg-1 leaf tissue) 1.53 ± 0.2 1.06 ± 0.28
  • a Values for polar leaf lipids are reported as the average (±SD) percentage of the total lipids. b n = No. of plants. Lipids were extracted from 100 mg of leaf tissue from 4-week-old plants that were selected on Murashige and Skoog agar and transferred to soil after 10 d. The transgenic plants were from the second generation (T2). Portions were removed for determination of lipid content by GC as described in “Materials and Methods.” Approximately 10 μg of lipids was injected onto the HPLC column. The conditions for separation are also described in “Materials and Methods.” Each sample was injected twice. Chlorophyll analysis was run separately on leaf samples from the same plants using the method of Lichtenthaler (1987).