Table III.

Bacterial strains and plasmids

Strain or Plasmid Relevant Characteristics Source
Bacterial strains
    HMS174 (DE3) F- recA1 hsdR(rK12- mK12+) RifR Novagen (Madison, WI)
    BL21trxB (DE3) F- ompT hsdSB (rB- mB-) gal dcm trxB15::kan Novagen
Plasmids
    pET23a(+) Expression vector providing a C-terminal hexahistidine tag Novagen
    pET23-Tkl Plasmid expressing a C-terminally His-tagged pseudomature TK This study
    pET32a(+) Expression vector providing a thioredoxin domain, hexahistidine tag, an S-Tag, and an enterokinase cleavage site Novagen
    pET32-Tkl7otp Plasmid expressing a thioredoxin-TK-fusion protein This study
    pET32-ZMTK- Plasmid expressing an engineered thioredoxin-TK-fusion protein This study
Tb