Table II.

Cytokinin content of petunia corollas following pollination (hap) and ethylene treatment (het)

Six corollas were collected from IPT22, IPT34, and WT flowers. Total cytokinins included Z, zeatin; ZR, zeatin riboside; iP, isopentenyl adenine; iPA, isopentenyl adenosine; Z-9-G, zeatin-9-glucoside; diHZ, dihydrozeatin; diHZ-9G, dihydrozeatin-9-glucoside; diHZR, dihydrozeatin riboside; and iP-9G, isopentenyl-9-glucoside.

Treatment Plant Line Total Cytokinins Z ZR iP iPA
ng g-1 dry wt
12 het WT 50.24±3.3a ndb 8.12±0.28 12.6±1.93 9.72±1.14
IPT22 2,830.45±626.1 758.74±225.6 2,014.85±711.65 12.65±1.49 15.11±0.25
IPT34 2,442.39±366.5 789.34±95.73 1,597.76±331.4 14.64±0.38 20.76±0.30
0 hap WT 24.55±1.1 nd 6.83±0.30 6.66±0.28 6.68±0.42
IPT22 38.36±2.5 nd 7.25±0.40 10.48±0.62 14.71±1.46
IPT34 43.63±2.5 nd 5.41±0.32 11.35±0.88 17.32±1.12
12 hap WT 31.07±0.7 nd 4.03±0.37 12.85±0.57 4.03±0.34
IPT22 49.93±0.3 nd 12.32±0.72 9.98±0.03 6.82±0.43
IPT34 41.54±4.0 nd 3.66±0.78 13.13±1.08 10.56±1.06
18 hap WT 20.55±0.9 nd 3.7±0.72 9.34±0.15 5.1±0.44
IPT22 176.62±12.6 31.12±4.46 118.02±6.19 11.17±0.82 11.15±0.86
IPT34 33.65±1.4 2.63±0.64 8.35±0.39 10.15±0.21 9.11±0.24
24 hap WT 29.83±0.9 nd nd 11.0±0.18 2.95±0.13
IPT22 323.58±33.8 162.22±29.51 126.85±15.71 9.84±0.74 8.58±0.79
IPT34 80.24±3.5 24.2±0.51 35.33±2.7 10.65±1.72 7.11±0.81
36 hap WT 19.35±1.9 nd nd 10.76±0.99 5.35±0.57
IPT22 61.7±2.8 4.43±0.26 20.17±1.27 15.83±1.04 14.94±1.49
IPT34 74.53±1.5 6.59±0.67 31.81±1.5 14.29±0.81 17.51±0.35
48 hap WT 25.58±2.1 nd 1.18±0.13 11.11±0.82 1.59±0.17
IPT22 721.68±54.4 180.65±28.86 502.64±77.01 6.6±0.29 5.0±0.08
IPT34 689.85±25.2 260.8±39.89 390.4±52.0 7.32±1.23 7.04±0.87
72 hap WT 7.055±0.9 nd nd 4.75±0.89 4.41±0.37
IPT22 475.73±23.2 211.63±17.35 228.98±14.64 13.99±2.31 12.52±1.3
IPT34 254.78±20.9 112.46±6.75 127.05±17.87 5.26±0.74 5.54±2.06
  • a Values represent the average of three replicates ± se

  • b nd, Not detectable