Table I.

Functional classification of genes selectively expressed in pollen

One hundred-fifty genes identified as selectively expressed in pollen are classified based on established as well as putative functions. The first column gives The Arabidopsis Information Resource (TAIR) annotation and the second gives the expression value of the gene in pollen (weighted average of triplicates). In the third column, the lower confidence bound fold change (FC; average of the comparisons of pollen to the four vegetative tissues) is given. The fourth and fifth column depict the Affymetrix probe set (Probes set) and, if available, the TAIR locus (AGIID) assigned to this probe set. Within each category, genes are ranked by highest signal.

Annotation Signal Probe Set Arabidopsis Genome Initiative Identification (AGI ID)
Signal transduction (37)
    Putative protein kinase 4,728 76.8 17803_at At1g61860
    Putative receptor-like protein kinase 4,061 78.4 18205_s_at At4g39110
    Putative Pro extensin-like receptor kinase PERK7 4,010 20.3 17812_at At1g49270
    Putative calcium-dependent protein kinase CPK24 3,538 160.8 16806_s_at At2g31500
    Putative 4,5 PIP kinase 3,425 45.9 19788_at At1g01460
    Putative calmodulin 3,326 214.9 13415_at At4g03290
    Putative Leu-rich repeat transmembrane protein kinase AtPRKc 3,051 80.7 19047_at At2g07040
    Putative phosphatidylinositol/phophatidylcholine transfer protein 1,887 15.0 19052_at At2g18180
    Putative calcium-dependent protein kinase CPK14 1,511 250.6 20236_s_at At2g41860
    Putative protein kinase 1,083 16.9 18152_at At2g24370
    Putative Leu-rich repeat transmembrane protein kinase AtPRKd 1,026 17.2 19103_at At5g35390
    Calcium-dependent protein kinase-like protein CPK26 854 26.4 20241_at At4g38230
    Putative Ser/Thr protein kinase 725 21.7 14364_at At1g04700
    Putative protein kinase (cdc2 kinase homolog cdc2MaC, M. sativa) 670 48.9 16824_at At4g10010
    Putative GTP-binding protein 652 11.6 18107_at At2g33870
    Putative inositol polyphosphate 5′-phosphatase 636 8.8 19795_at At2g43900
    Putative proline extensin-like receptor kinase PERK5 611 29.0 16876_at At4g34440
    Putative protein (GTPase-activating protein, Yarrowia lipolytica) 574 4.9 16758_at At4g27100
    Putative inositol polyphosphate 5′-phosphatase 563 17.1 19582_at At2g31830
    Putative protein (various predicted protein kinases, Arabidopsis) 532 11.0 18193_at At5g26150
    AK20 gene protein kinase catalytic domain (fragment) 519 6.1 19111_at
    Putative receptor-like Ser/Thr kinase RKF2 483 10.4 17496_at At1g19090
    Putative Pro extensin-like receptor kinase PERK12 335 14.7 17359_at At1g23540
    Hypothetical protein (similar to wall-associated kinase 1, Arabidopsis) 324 10.8 18186_at At1g78940
    Putative Ptα kinase interactor (similar to Ptα kinase interactor 1, tomato) 300 3.8 18825_at At4g13190
    Putative GTP-binding protein 292 16.3 12489_at At2g22290
    Protein phosphatase 2C (PP2C) 275 4.8 19345_at At2g40180
    Putative calcium-dependent protein kinase CPK20 241 8.8 12311_at At2g38910
    Putative calmodulin (calmodulin PIR1:MCKM, Chlamydomonas reinhardii) 229 4.7 19274_i_at At4g12860
    Protein kinase-like protein 214 5.7 19556_at At4g31220
    Putative protein (various predicted protein kinases, Arabidopsis) 159 2.2 19071_at At5g35380
    Putative Ser/Thr protein kinase 110 4.0 16866_at At1g79250
    Putative STE20/PAK-like protein kinase (similar to yeast STE20) 74 4.7 18999_at At1g70430
    Putative receptor-like kinase 65 3.4 14275_at At4g31230
    Putative protein kinase 62 1.8 16848_at At2g20470
    Putative phosphatidylinositol-4-phosphate 5-kinase 55 4.3 19310_at At2g41210
    Calcium-dependent Ser/Thr protein kinase CPK18 44 1.8 16849_at At4g36070
Cell wall biosynthesis and regulation (16)
    Pectinesterase family (nearly identical to pollen-specific BP10 protein, canola) 7,180 110.3 17753_at At1g55570
    Exopolygalacturonase 7,059 51.9 18923_g_at At3g07850
    Extensin-like protein 6,221 71.5 15765_at At4g33970
    Putative polygalacturonase 5,660 91.2 15716_at At2g23900
    Putative cellulose synthase (AtCalD1—homolog of NaCalD1) 4,911 58.9 20243_at At2g33100
    Glycosyl hydrolase family 10 (xylan endohydrolase isoenzyme X-I, barley) 4,536 40.1 13393_at At4g33860
    Polysaccharide lyase family 1 (similar to pectate lyase P59, tomato) 4,226 184.1 16837_at At2g02720
    Polysaccharide lyase family 1 (At59) 3,914 83.0 16857_at At1g14420
    Putative cellulose synthase (AtCalD4—homolog of NaCalD1) 3,489 28.5 16782_at At4g38190
    Putative protein (bp4A protein, Brassica napus) 1,196 51.4 15360_at At4g11760
    COBRA-like protein COBL-11 929 13.1 15331_at At4g27110
    Cellose synthase (1,3-β-glucan synthase) family (AtGal2, homolog of NaGal1) 925 2.7 18515_at At2g13675
    Pectinesterase family 662 19.2 18519_at At4g33230
    Glycosyl hydrolase family 17 (BETAG5, β-1,3-glucanase bg5) 438 5.9 18539_at At5g20340
    Putative xyloglucan endotransglycosylase 214 5.8 19530_at At4g18990
    Glycosyl hydrolase family 17 (BETAG4, β-1,3-glucanase bg4) 214 2.3 18120_i_at At5g20330
Stress response (11)
    Putative plant defensin protein PDF2.6 9,739 53.2 19928_at At2g02140
    Putative peroxiredoxin (similar to type 2 peroxiredoxin, Brassica rapa) 3,640 7.3 15966_i_at At1g60740
    17.6-kD heat shock protein (AA 1-156) 1,637 26.5 13276_at At1g53540
    Heat shock protein 17.6A (At-HSP17.6A) 731 17.2 13278_f_at At5g12030
    Heat shock protein 22.0 (At-HSP22.0) 431 8.6 17076_s_at At4g10250
    Putative protein (similar to Mlo proteins, barley) 402 4.8 14639_at At2g33670
    Heat shock protein 18 299 3.1 13280_at At5g59720
    Putative stress protein 130 6.1 13378_at At2g01330
    Putative peroxidase 108 4.4 17828_at At4g17690
    Mitochondrion-localized small heat shock protein (AtHSP23.6-mito) 69 2.0 13282_s_at At4g25200
    Putative disease resistance protein (TIR-NBS-LRR class) 61 2.8 12258_at At4g14370
Metabolism (9)
    Glycosyl hydrolase family 35 (β-galactosidase) BGAL11 6,152 194.9 16874_at At4g35010
    Glycosyl hydrolase family 32 (invertase) 3,759 54.4 16369_s_at At3g52600
    Glycosyl hydrolase family 35 (β-galactosidase) BGAL13 3,074 69.7 17316_at At2g16730
    FAD-linked oxidoreductase family 2,481 126.4 15302_at At1g11770
    Putative thioredoxin 2,236 21.2 19937_at At2g33270
    Putative acyl-CoA:1-acylglycerol-3-phosphate acyltransferase 656 10.4 18423_at At1g51260
    3-hydroxy-3-methylglutaryl CoA reductase HMG1 isoform HMGR1L 550 3.0 20160_at
    Glycosyl hydrolase family 1 101 18.6 19601_at At2g25630
    Putative CDP-diacylglycerol synthetase 53 1.7 19016_at At4g26770
Ion transport (8)
    Putative cation/H+ antiporter AtCHX8 3,259 17.1 17246_at At2g28180
    Putative protein (anion exchange protein 2, Homo sapiens) 3,208 67.1 18837_at At4g32510
    Shaker potassium channel SPIK 1,489 51.8 16783_at At2g25600
    Putative cation/H+ antiporter AtCHX15 618 4.3 14334_at At2g13620
    Ion transporter-like protein AtNRAMP5 404 30.2 18118_at At4g18790
    Putative cyclic nucleotide-regulated ion channel AtCNGC16 205 8.3 14291_at At3g48010
    G subunit of vacuolar-type H+-ATPase (vag1) 78 5.3 16226_at At4g25950
    Putative cation/H+ antiporter AtCHX13 60 2.4 15704_at At2g30240
Cytoskeleton (8)
    Actin 4 3,262 110.7 14356_at At5g59370
    Actin depolymerizing factor-like protein 3,092 37.4 12529_at At4g25590
    Actin 12 1,815 35.8 15697_at At3g46520
    Prolilin 4 2,691 25.8 12411_s_at At2g19770
    Myosin heavy chain-like protein AtVIIIB 341 19.7 12257_at At4g27370
    Putative myosin heavy chain AtXID 224 2.7 16327_at At2g33240
    Putative myosin II heavy chain (Naegleria fowleri) 179 6.2 15329_at At4g33390
    Kinesin-related protein 97 3.3 16794_at At1g09170
Transcriptional regulation (5)
    Putative protein (NorM, Vibrio parahaemolyticus) 1,471 33.5 14992_at At4g21900
    MYB97 (similar to anther-specific myb-related protein 2, tobacco) 309 10.4 17979_at At4g26930
    MADS-box protein AGL30 (homolog of MADS1;11, tobacco) 167 5.2 18443_at At2g03060
    MADS-box protein AGL29 154 15.8 19309_at At2g34440
    CONSTANS B-box zinc finger family protein 23 1.9 15698_at At4g15250
Proteolysis (5)
    Putative Ser carboxypeptidase II 7,644 55.0 17765_at At2g05850
    Ser-type carboxypeptidase-like protein 4,000 117.4 18137_at At3g52010
    Subtilisin-like Ser protease 1,348 26.8 16349_at At4g21326
    Ser-type carboxypeptidase-like protein 721 12.1 17814_at At3g52000
    Ser-type carboxypeptidase-like protein 64 6.4 18461_at At3g52020
Vesicle trafficking (5)
    Syntaxin AtSYP124 1,243 10.7 15275_at At1g61290
    ARF GTPase-activating domain-containing protein 829 15.2 12639_at At2g35210
    Clathrin protein family 631 8.9 13499_at At1g05020
    ARF GTPase-activating domain-containing protein 598 4.4 17446_at At2g14490
    Unknown protein (similar to yeast Sec7p protein) 280 7.9 18381_at At2g30690
Sugar and protein transport (3)
    Monosaccharide transporter 4,441 129.6 19270_at At5g23270
    Putative ABC transporter AtNAP12 437 4.6 13049_at At2g37010
    Putative vacuolar sorting receptor 182 2.8 18509_at At2g30290
Transposition (3)
    Ac-like transposase (related to Ac/Ds transposon family of maize) 198 4.8 16730_at At2g16040
    Putative Mutator-like transposase 118 2.1 12987_s_at At2g30640
    Putative TNP2-like transposon protein 60 2.6 19339_i_at At2g10140
Water channel (2)
    Aquaporin-like protein TIP5;1 (aquaporin, Vernicia fordii) 1,497 13.6 18856_at At3g47440
    Putative water channel protein—major intrinsic protein (MIP) TIP1;3 544 6.1 17798_at At4g01470
Translational regulation (2)
    Putative protein (similar to polyadenylate-binding protein PABP) 429 10.9 16836_at At3g16380
    Putative translation initiation factor 65 5.2 18609_at At2g39820
Cell cycle regulation (1)
    Cyclin A2;1 89 1.8 16851_s_at At5g25380
Unknown and hypothetical (35)
    Arabinogalactan-protein AGP6 16,351 50.8 12003_at At5g14380
    Nectarin-like protein (nectarin I precursor, Nicotiana plumbaginifolia) 11,576 81.8 19014_at At5g26696
    Hypothetical protein 9,322 89.6 16201_at At1g49490
    Unknown protein (homolog of protein ARPL 1;1) 9,259 181.8 19385_at At1g54070
    Putative LIM-domain protein (AtPLIM2) 8,733 82.0 20478_at At2g45800
    Unknown protein 8,161 92.0 19301_at At4g36490
    Unknown protein 7,359 35.4 12568_at At2g33420
    Putative pistil-specific protein (similar to potato pistil-specific protein STS15) 7,018 149.3 14936_at At4g02250
    Hypothetical protein 4,933 47.1 13912_at At1g04540
    Unknown protein 3,744 101.5 12638_at At1g04470
    Hypothetical protein 3,186 61.3 18426_at At2g13350
    Unknown protein 1,225 42.7 17230_at At2g40990
    Unknown protein 962 96.2 20549_i_at At2g26850
    Hypothetical protein 818 33.4 17687_at At1g60240
    Putative GDP-dissociation inhibitor 753 28.9 19790_at At1g12070
    Hypothetical protein 466 5.4 15925_at At1g43630
    Unknown protein 420 13.4 13836_at At1g06750
    Unknown protein 379 27.4 15838_at At2g29790
    Hypothetical protein 377 2.7 13304_at At1g24330
    Putative protein (MSP1, Saccharomyces cerevisiae) 348 21.5 19604_at At4g28000
    Unknown protein (related to MO25, early mouse development protein family) 329 28.7 17711_at At2g03410
    RALF-LIKE 10 313 7.0 18385_at At2g19020
    Hypothetical protein 258 6.9 13814_at At2g29040
    Unknown protein 254 5.8 15277_at At2g41440
    Glu-rich protein 238 22.1 16656_at At4g20160
    Putative protein 227 18.3 15322_at At4g24580
    Unknown protein 221 7.6 19849_at At2g18080
    Unknown protein 219 4.3 14496_at At2g45610
    Hypothetical protein 155 4.7 14379_at At2g33320
    Unknown protein 128 2.6 18454_g_at At4g08605
    Unknown protein 75 1.9 15016_at At1g04090
    Putative protein 51 2.1 12076_at At4g36600
    Hypothetical protein 49 2.3 15266_at At4g08630
    Hypothetical protein 45 3.5 15262_at At2g33190
    Putative protein, (BRCA1-associated RING domain protein, H. sapiens) 32 1.4 17790_g_at At4g21070