Table VI.

Relative expression level of M. truncatula transcripts based on the repetitive occurrence of sequences in the EST data sets from immature seeds

The cluster accession nos. of the M. truncatula ESTs identified by MS were extracted from the Medicago EST Navigation System (http://medicago.toulouse.inra.fr/) release of January 2003. The cluster accession nos. were used to search the no. of sequences from the same mRNA in two different data sets: a cDNA library of immature seeds ranging in age from 11 to 19 DAP (MtGESD, 4,525 ESTs) and a cDNA library of immature seeds ranging in age from 25 to 35 DAP (MtGLSD, 4,866 ESTs). The M. truncatula transcripts whose expression was found to be significantly variable in the two different data sets (χ2 test, P < 0.050) were grouped according to their expression profiles during seed development. In each class of proteins (see Table I), the corresponding M. truncatula transcripts were sorted in the descending order based on their EST counts in both data sets.

Protein Class Strongest BLAST Hit EST gi Cluster Accession No. MtGESD (11-19 DAP) MtGLSD (25-35 DAP)
Transcripts preferentially expressed during early stages of seed development
    1 Vicilin 14986367 MtC60032.3_GC 282 55
    1 Vicilin 14984555 MtC60159_GC 41 21
    1 Chlorophyll a/b binding 13783768 MtC00119_GC 10 0
    2 Vicilin 14984749 MtC60032.4_GC 280 55
    2 Annexin 7718287 MtC20316_GC 14 1
    2 S-adenosylmethionine synthetase 11609660 MtC00046_GC 4 0
    3 Flavonone 3-hydroxylase 13596069 MtC93321_GC 12 0
    3 Protein disulfide isomerase 10697964 MtC10403_GC 10 0
    3 GAPDHc 9671653 MtC00021.1_GC 4 0
Transcripts preferentially expressed during late stages of seed development
    1 Legumin 14985010 MtC60042_GC 119 451
    1 Legumin 14986270 MtC60076_GC 67 311
    1 Convicilin 14985541 MtC60090_GC 71 209
Transcripts whose relative levels did not vary significantly during seed development
    1 Lipoxygenase 14982738 MtC60669_GC 11 15
    1 ACC oxidase 10520454 MtC10108.2_GC 9 6
    1 Met synthase 9669628 MtC00018_GC 4 6
    1 Homology not assigned 13595945 MtD00176_GC 1 2
    1 Glyoxalase 1 11897629 MtC60084_GC 3 0
    1 Oxygen-evolving enhancer 11902222 MtC60015_GC 2 1
    1 Starch synthase 13379977 MtC90709_GC 2 1
    1 Reversibly glycosylated polypeptide-1 7718398 MtC10969_GC 2 0
    1 110-kD 4SnNc-Tudor 7564626 MtC50269.1_GC 1 1
    1 Triosephosphate isomerase 13380542 MtC00059_GC 1 0
    1 Thiamine biosynthesis protein 7718895 MtD00077_GC 1 0
    1 Lipoxygenase 6708795 MtC60306_GC 0 0
    1 S-adenosyl-l-homo-Cys hydrolase 1710838 MtC30011_GC 0 0
    2 β-Tubulin 7561503 MtC00356.1_GC 2 1
    2 Adenosine kinase 10704292 MtC10064_GC 1 0
    2 Pyruvate DH β-subunit 13780026 MtC30080_GC 0 0
    2 Myo-inositol-1-P synthase 13369792 MtC60071_GC 0 0
    3 Heat shock cognate protein 80 14882556 MtD00014_GC 5 1
    3 Molecular chaperone BiP A 14983890 MtC00550.1_GC 3 0
    3 Heat shock protein 90 10706885 MtC00079_GC 3 0
    3 Putative homology not assigned 9678016 MtC30333_GC 0 0
    3 Resistance gene analog protein 13779916 MtD02701_GC 0 0
    4 Elongation factor EF-2 10698429 MtC00166.1_GC 2 0