Table III.

Metabolite levels in illuminated leaves of 6-week-old plants of the tomato genotypes

Leaves were harvested 6 h into the photoperiod and irrespective of the method of analysis, all metabolite analyses were performed from the same tissue samples. Values presented are the mean ± se of measurements from six plants per genotype. Amino acid values in parentheses were determined in samples taken in the dark period. Organic acid contents, normalized as described in “Materials and Methods,” are also presented as the mean ± se of measurements from six plants per genotype. Asterisks and bold type indicates those that were determined by the Student's t test to be significantly different between genotypes.

Metabolite Lp Aco-1
Organic acids
    Succinate 1.0 ± 0.3 0.4 ± 0.1*
    Isocitrate 1.0 ± 0.3 1.4 ± 0.3*a
    Citrate 1.0 ± 0.1 1.5 ± 0.1*
    α-kt-glutarate 1.0 ± 0.4 0.3 ± 0.4*
    Malate 1.0 ± 0.5 0.9 ± 0.2
    Fumarate 1.0 ± 0.1 0.5 ± 0.1*
nmol g fresh wt-1
Amino acids
    Asp 308.2 ± 29.0 (222.9 ± 3.3) 149.9 ± 12.2* (182.9 ± 33.4)
    Glu 732.8 ± 207.5 729.5 ± 75.4 (476.3 ± 112.7)*
(737.9 ± 91.7)
    Asn 141.5 ± 16.5 (163.8 ± 24.3) 228.2 ± 40.6* (105.9 ± 19.7)
    Ser 138.6 ± 22.8 (144.9 ± 19.3) 166.1 ± 40.1 (105.8 ± 19.1)
    Gln 264.3 ± 59.9 (259.3 ± 50.8) 206.1 ± 51.4* (164.6 ± 47.0)*
    Gly 98.4 ± 26.82 (n.d.b) 184.3 ± 30.9* (n.d.)
    Arg 124.9 ± 29.1 (148.7 ± 4.5) 100.1 ± 9.9 (72.2 ± 11.3)*
    Phe 49.6 ± 18.7 (46.6 ± 9.4) 28.5 ± 1.9* (22.7 ± 6.3)*
    Leu 32.5 ± 11.1 (11.7 ± 1.9) 19.3 ± 0.9* (8.5 ± 1.3)
Nucleotides
    ATP 11.7 ± 2.7 19.7 ± 2.0
    ADP 21.3 ± 3.1 44.0 ± 11.5*
    ATP/ADP 0.6 ± 0.0 0.4 ± 0.0*
Σ Adenylate 32.3 ± 7.0 59.7 ± 18.3*
UDP-Glc 4.7 ± 0.8 15.6 ± 5.6*
    UTP 15.9 ± 2.5 17.7 ± 3.9
    UDP 4.7 ± 0.9 0.37 ± 0.0*
    UTP/UDP 3.7 ± 0.5 30.0 ± 3.6*
    ATP/UTP 0.9 ± 0.2 1.0 ± 0.2
Sugar phosphate
    3 phosphoglycerate (3PGA) 262.0 ± 23.2 316.0 ± 16.8*
    Glucose 6-phosphate 106.4 ± 4.9 137.2 ± 5.6*
    Glucose-1-phosphate 19.6 ± 3.3 25.1 ± 2.2
    Fructose 6-phosphate 19.8 ± 1.6 24.7 ± 4.3
    Σ hexose-P 145.8 ± 8.5 187.0 ± 9.9*
    Pi 5.3 ± 1.8 5.8 ± 0.6
    Σ Phosphates (inorganic phosphate) Pi 75.5 ± 1.3 86.9 ± 2.1*
    3PGA/Pi 48.6 ± 5.7 54.6 ± 3.95*
  • a Isocitrate was measured following the protocol described by Beutler (1989).

  • b n.d., Not detectable.