Table II.

Mass spectrometric identification of spots on two-dimensional gels

Spot No. Database Gene Identifier Annotation MOWSE (Molecular Weight Search) Score Peptides Matched (Total No. of Peptides) Predicted Molecular Mass (D) Predicted pl Coveragea Codine Site Plastid Targetingb
%
3317 National Center for Biotechnology Information (NCBI) 134102 60-kD chaperonin alpha subunit (Cpn60), chloroplast precursor 5.42 E + 03 7 (32) 57,521 4.83 13 Nucleus Yes
3603 NCBI 134102 60-kD chaperonin alpha subunit (Cpn60), chloroplast precursor 5.42 E+03 7 (32) 57,521 4.83 13 Nucleus Yes
3610 NCBI 134102 60-kD chaperonin alpha subunit (Cpn60), chloroplast precursor 1.00 E+04 7 (22) 57,521 4.83 16 Nucleus Yes
3615 NCBI 134102 60-kD chaperonin alpha subunit (Cpn60), chloroplast precursor 1.60 E+05 9 (31) 57,521 4.83 18 Nucleus Yes
3324 The Institute for Genomic Research (TIGR) Zm TC88712 20-kD chaperonin (Cpn20), chloroplast precursor 9.88 E+5 8 (25) 27,095 6.25 37 Nucleus Yes
4209 TIGR Zm TC88576* 20-kD chaperonin (Cpn20), chloroplast precursor 1.02 E+7 8 (19) 26,569 8.48 45 Nucleus Yes
3320 NCBI 15912247 or 13926292 33-kD subunit of OEC, PSI 93.4 4 (22) 35,128 5.55 11 Nucleus Yes
3330 NCBI 15408655 33-kD subunit of OEC, PSI (pulative) 984 4 (25) 34,861 6.09 17 Nucleus Yes
4305 TIGR Zm TC81083 33-kD subunit of OEC, PSII 2.13 E+6 9 (26) 37,531 5.92 43 Nucleus Yes
6537 TIGR Zm TC88557 Acetyl CoA carboxylase 214 5 (22) 258,314 5.93 2 Nucleus Yes
7537 NCBI 7438099 Acetyl CoA carboxylase 1.25 E+3 9 (32) 252,131 5.91 6 Nucleus No
7616 TIGR Zm TC90687 ATPase subunit alpha 5.48 E+5 8 (31) 55,707 5.86 23 Plastid N.A.c
8632 TIGR Zm TC90687 ATPase subunit alpha 1.36 E+5 8 (26) 55,707 5.86 19 Plastid N.A.
8633 TIGR Zm TC90687 ATPase subunit alpha 2.87 E+5 7 (30) 55,707 5.86 19 Plastid N.A.
1246 TIGR Zm TC85581 ATPase subunit beta 1.69 E+7 11 (29) 54,041 5.31 28 Plastid N.A.
4520 NCBI 629818 ATPase subunit beta 7.15 E+4 7 (16) 59,249 5.56 18 Plastid N.A.
4533 NCBI 552857 ATPase subunit beta 5.82 E+5 9 (23) 53,954 5.30 19 Plastid N.A.
4534 NCBI 629818 ATPase subunit beta 1.54 E+5 7 (14) 59,249 5.56 20 Plastid N.A.
5504 NCBI 552857 ATPase subunit beta 3.52 E+3 5 (24) 53,954 5.30 17 Plastid N.A.
5507 NCBI 629818 ATPase subunit beta 7.23 E3 6 (22) 59,249 5.56 14 Plastid N.A.
5517 NCBI 6815115 ATPase subunit beta 4.63 E+5 11 (30) 53,997 5.38 23 Plastid N.A.
5518 NCBI 629818 ATPase subunit beta 1.14 E+3 5 (19) 59,149 5.56 11 Plastid N.A.
5725 NCBI 874823 ATPase subunit beta 4.86 E+4 7 (32) 53,717 5.20 18 Plastid N.A.
6511 NCBI 552857 ATPase subunit beta 2.54 E+5 9 (19) 53,954 5.30 24 Plastid N.A.
6708 TIGR Zm TC85581 ATPase subunit beta 3.69 E+6 12 (28) 54,041 5.31 30 Plastid N.A.
201 TIGR Zm TC86875 ATPase subunit delta 1.22 E+4 5 (17) 20,206 4.35 38 Nucleus Yes
6439 TIGR Zm TC88574 ATPase subunit gamma 104 4 (17) 42,529 8.80 11 Nucleus Yes
7414 TIGR Zm TC88574 ATPase subunit gamma 1.21 E+3 7 (14) 42,529 8.83 18 Nucleus Yes
8520 NCBI 7489718 Beta-D-glucosidase, glu2 precursor 1.74 E+2 7 (16) 64,112 6.72 16 Nucleus Yes
%
5406 TIGR Zm TC85027 Chloroplast NADP-malate dehydrogenase 9.64E+3 6 (30) 49,481 6.62 17 Nucleus Yes
6705 NCBI 5360574 ClpC protease 5.16E+3 6 (19) 105,740 6.06 8 Nucleus Yes
6711 NCBI 2921158 ClpC protease 3.95E+7 14 (26) 103,456 6.27 14 Nucleus Yes
3632 NCBI 16444957 Cryptochrome 1 1.05E+3 5 (20) 79,300 5.37 9 Nucleus No
4738 NCBI 16444957 Cryptochrome 1 631 4 (26) 79,301 5.37 8 Nucleus No
4741 NCBI 16444957 Cryptochrome 1 2.63E+3 5 (32) 79,300 5.37 10 Nucleus No
4417 NCBI 15809970 Enolase (2 phospho D glycerate hydroylase) 1.47E+3 5 (31) 47,704 5.74 18 Nucleus No
2714 NCBI 6746592 Hsp70 9.32E+3 7 (33) 77,106 5.13 10 Nucleus Yes
2720 NCBI 6746592 Hsp70 5.55E+4 8 (27) 77,106 5.13 11 Nucleus Yes
329 NCBI 100903 Nucleic acid-binding protein (NABP) 1.51E+3 4 (24) 33,117 4.60 18 Nucleus Yes
4409 TIGR Zm TC82882 Phosphoglycerate kinase, chloroplast precursor 2.44E+4 5 (28) 24,114 9.12 33 Nucleus Yes
4219 TIGR Zm TC89102 Plastid-specific ribosomal protein “2” 1.08E+5 8 (17) 26,232 8.46 25 Nucleus Yes
5421 NCBI 13489165 Beta amylase 3.37E+3 8 (32) 57,953 6.74 19 Nucleus Yes
6441 TIGR Zm TC82049 SU1 isoamylase 468 4 (21) 91,619 5.85 6 Nucleus Yes
7313 TIGR Zm TC81138 Sucrose synthase (UDP-glucose: D fructose-2-glucosyl-transferase) 152 4 (9) 93,696 6.18 4 Nucleus No
6402 TIGR Zm TC84809 Glyceraldehyde-3-phosphate dehydrogenase 3.62E+4 5 (25) 46,952 5.95 18 Nucleus Yes
3629 NCB1 12325133 Unknown protein 193 6 (25) 66,659 5.06 12 Nucleus No
7230 NCB1 16905193 Hypothetical protein 3.09E+2 4 (23) 26,072 6.34 21 Nucleus No
1353 NCBI 7459088 Hypothetical protein 4.05E+3 4 (37) 11,782 4.94d 28 Nucleus No
3331 NCBI 16604341 Unknown protein 2.36E+3 5 (20) 39,424 5.12d 13 Nucleus No
1145 TIGR Zm TC81772 Inosinc monophosphate dehydrogenase 3.65E+4 6 (26) 11,784 9.78d 61 Nucleus No
2528 NCBI 3915111 Cytochrome P450 (C4H) 1.5 E+3 5 (23) 58,011 9.05d 16 Nucleus No
3602 NCBI 15554512 Male sterility 1 protein 1.40 E14 7 (32) 76,974.2 7.82d 17 Nucleus No
4301 TIGR Zm TC89587 Mitotic spindle checkpoint protein MAD2 2.61 E4 4 (21) 26,664 4.73d 24 Nucleus No
6217 TIGR Zm TC89879 Putative protein phosphatase 1.03E+3 4 (37) 25,946 10.38d 25 Nucleus No
  • a Percent of predicted protein sequence the peptides matched. b Plastid targeting sequence determined by ChloroP software. c N.A., Not applicable, protein is plastid encoded. d Predicted pl does not match the pl of the spot on the gel.