Table II.

AtTLP gene expression analysis via searching microarray expression databasea

Ch2/Ch1 Normalized (Mean)bc
ExperimentChannel 1 DescriptionChannel 2 DescriptionAtTLP2AtTLP7AtTLP9AtTLP10
Hormone Effect
    Auxin responsemsgd seedlings, untreatedmsg seedlings; 10 μm indole-3-acetic acid for 30 min0.322.21
    Auxin inductionMock-treated Col-0 rootsNaphthylacetic acid-treated Col-0 roots0.462.22
    Cytokinin responseControl15 min of cytokinin treatment2.19
    Abscisic acid insensitive 1Wild-type controlAbscisic acid insensitive 1 mutant0.492.60.35
    edr1 mutantWild-type leavesedr1 mutant leaves2.33
    Downstream genes of KN1ControlOverexpression of KN1-GR in Col-0 background0.432.784.73
Stress
    Effects of elevated atmospheric CO2Col-0 leaves 360 ppm CO2Col-0 leaves 1,000 ppm CO20.33
    Genes involved in chilling toleranceCold-treated Col-0 wild-type tissueCold-treated cls8 mutant tissue0.220.15
    Genes involved in potassium nutrition[K+] = 120 μm[K+] = 2 mm0.340.2
    CadmiumControl10 μm cadmium-treated plant2.72
  • a These data are obtained from http://genome-www5.stanford.edu.

  • b All data are corresponding with fluorescence intensities greater than 500 in both channels and ch2:ch1 normalized ratio ≧2.0 or ≦0.5.

  • c When searching dbEST with BLASTN, we find Arabidopsis EST corresponding to four of our AtTLPs represented on microarray data generated by AFGC. AtTLP2 is corresponding to the EST clones 289B10T7 and 173K22T7. AtTLP7, AtTLP9, and AtTLP10 are corresponding to the EST clones 173G1T7, 201E19T7, and F3E6T7, respectively.

  • d It is the mutant of massugu gene, which has meanwhile been officially renamed to NPH4 (nonphototropic hypocotyl 4) gene.