Table I.

Transcripts elevated in leaves of Arabidopsis plants subjected to a combination of drought and heat stress

Gene No.Transcript NameAvg.sd
Section A
At3g46230Heat shock protein 179.71.7
At3g12580Heat shock protein 707.51.5
At5g48570Peptidylprolyl isomerase5.90.4
At5g59220ABA-induced protein phosphatase 2C5.30.2
At3g24500Ethylene-responsive transcriptional coactivator4.30.4
At2g47180Galactinol synthase3.80.3
At3g53230CDC48-like endoplasmic reticulum ATPase3.60.5
At3g16360Putative two-component phosphorelay mediator3.00.2
At1g65660Step II splicing factor SLU72.90.1
At3g24520Heat shock transcription factor HSF12.90.2
At3g07770Putative heat shock protein2.60.3
At1g22770Putative gigantea protein2.50.1
At4g22590Trehalose-6-phosphate phosphatase2.50.2
At5g28540Luminal binding protein2.10.1
Section B
At5g59720Heat shock protein 1810.00.6
At4g25200Mitochondrion-small heat shock protein9.90.6
At4g27670Heat shock protein 219.50.3
At1g5354017.6-kD Heat shock protein9.01.2
At5g12020Heat shock protein 17.6-II8.90.4
At5g12030Heat shock protein 17.6A8.31.8
At4g10250Heat shock protein 22.07.31.3
At2g29500Putative small heat shock protein6.50.2
AtMg00520Maturase5.81.3
At3g09640Putative ascorbate peroxidase5.70.6
At1g74310Heat shock protein 101 (HSP101)5.50.3
At5g12110Elongation factor 1B alpha-subunit5.10.7
At4g03320Putative chloroplast import component5.00.5
At5g52640Heat shock protein5.00.1
At1g62510Similar to 14-kD Pro-rich protein4.70.1
AtMg00160Cytochrome c oxidase subunit 24.40.3
At1g72660Putative GTP-binding protein4.40.3
At2g3212070-kD Heat shock protein4.20.2
AtMg00513NADH dehydrogenase subunit 54.20.3
AtMg00270NADH dehydrogenase subunit 64.10.5
AtMg01360Cytochrome c oxidase subunit 13.80.2
At2g20560Putative heat shock protein3.50.3
At4g18280Gly-rich cell wall protein3.50.1
AtMg00650NADH dehydrogenase subunit 4L3.40.1
At5g37670Cytosolic class I small heat shock protein3.30.1
AtMg00960Cytochrome c biogenesis3.30.2
At1g07350Transformer-SR ribonucleoprotein3.20.2
At5g25450Ubiquinol–cytochrome-c reductase3.20.2
At3g48520Cytochrome P450-like protein2.70.1
At1g26580Putative MYB family transcription factor2.70.3
At5g67080Protein kinase-like protein2.70.7
At1g09140Putative SF2/ASF splicing modulator2.60.1
At5g58590Ran binding protein 1 homolog2.60.1
AtMg00090Ribosomal protein L162.50.1
At3g13470Chaperonin 60 beta2.50.1
At1g09950Similar to Nicotiana tumor-related protein2.40.1
At3g04000Short-chain type dehydrogenase/reductase2.40.1
AtMg01080ATP synthase subunit 92.30.1
At4g36010Thaumatin-like protein2.30.1
At3g59350Pto kinase interactor 12.30.1
At5g14800Pyrroline-5-carboxylate reductase2.20.1
At5g58070Outer membrane lipoprotein2.20.1
At1g56340Calreticulin (crt1)2.10.1
AtMg00180Cytochrome c biogenesis2.00.1
Section C
At5g06760Late embryogenesis abundant protein8.90.1
At5g59310Nonspecific lipid-transfer protein precursor8.40.3
At1g22990Putative metal-binding protein8.01.0
At5g13170Senescence-associated protein (SAG29)7.50.7
At3g02480Unknown protein similar to pollen coat protein7.20.8
At1g17020Fe(II)/ascorbate oxidase6.60.6
At5g59320Nonspecific lipid-transfer protein precursor6.50.1
At1g52690Late embryogenesis-abundant protein6.10.2
At5g66400Dehydrin RAB18-like protein5.70.1
At3g17520Unknown protein identical to LEA-like protein5.30.6
At1g05100Putative NPK1-related MAP kinase5.10.2
At1g07430Protein phosphatase 2C4.90.5
At5g52300Low-temperature-induced 65-kD protein4.81.0
At2g46680Homeodomain transcription factor (ATHB-7)4.60.3
At5g29000Putative CDPK substrate protein 14.30.7
At3g22840Early light-induced protein4.30.3
At1g64660Met/cystathionine gamma lyase4.20.3
At5g56500Rubisco chaperonin, 60 kD4.10.4
At5g25110Ser/Thr protein kinase-like protein4.00.8
At3g60350Arm repeat containing protein3.70.7
At1g70300Potassium transporter3.70.1
At1g02205Lipid transfer protein3.70.3
At2g15480Putative glucosyltransferase3.60.7
At1g56600Water stress-induced protein3.60.2
At1g54100Aldehyde dehydrogenase3.50.1
At5g05410DREB2A3.50.2
At4g15910Drought-induced protein-like3.50.2
At1g03940Anthocyanin 5-aromatic acyltransferase3.50.2
At1g62570Flavin-containing monooxygenase3.40.3
At4g33150Lys-ketoglutarate reductase/saccharopine3.40.2
At1g02205Receptor-like protein glossy13.40.4
At1g57590Pectinacetylesterase precursor3.30.2
At1g61800Glucose-6-phosphate/phosphate-translocator3.30.2
At2g43590Putative endochitinase3.30.1
At5g37540Nucleoid DNA-binding protein3.20.1
At1g62290Aspartic protease3.20.1
At5g53870Phytocyanin/early nodulin-like protein3.20.1
At5g18820Chaperonin 60 alpha chain3.10.7
At1g06430FtsH zinc dependent protease3.10.3
At4g35940Putative Glu-rich protein3.10.9
At5g52310Low-temperature-induced protein 783.10.2
At3g57520Probable imbibition protein3.10.1
At2g46270G-box binding bZIP transcription factor3.10.3
At1g21400Alpha keto-acid dehydrogenase3.10.1
At4g20320CTP synthase-like protein3.00.8
At3g55610Delta-1-pyrroline-5-carboxylate synthetase3.00.1
At2g33380Putative calcium-binding EF-hand protein3.00.1
At2g33590Putative cinnamoyl-CoA reductase2.90.1
At2g21590Putative ADP-glucose pyrophosphorylase2.90.3
At5g09590Heat shock protein 70 (Hsc70-5)2.90.3
At3g57540Putative DNA binding protein2.80.2
At4g24000Cellulose synthase catalytic subunit2.80.7
At5g14270Kinase-like RING3 protein2.80.3
At1g73040Jacalin2.80.4
At4g35580NAM/CUC2-like protein2.80.5
At3g13672Seven in absentia-like protein2.80.2
At2g04030Putative heat shock protein2.70.1
At1g51140Similar to phytochrome interacting factor 32.70.1
At3g61890Homeobox-Leu zipper protein ATHB-122.70.2
At1g56650Anthocyanin22.70.2
At5g04530Fatty acid elongase 3-ketoacyl-CoA synthase2.60.4
At3g50970Dehydrin Xero22.60.1
At4g12280Copper amine oxidase-like2.60.2
At3g05650Putative disease resistance protein2.60.1
At2g16890Putative glucosyltransferase2.60.4
At1g52890Similar to NAM (no apical meristem)2.60.0
At2g18050Histone H12.60.2
At3g05640Putative protein phosphatase-2C2.60.3
At4g21650Subtilisin proteinase2.50.3
At1g11480Similar to eukaryotic initiation factor 4B2.50.1
At5g22290NAM (no apical meristem)-like protein2.50.4
At1g72770Protein phosphatase 2C (AtP2C-HA)2.50.2
At3g05660Putative disease resistance protein2.50.4
At5g57050Protein phosphatase 2C ABI22.50.3
At5g40760Glucose-6-phosphate dehydrogenase2.50.3
At4g34000ABA responsive elements-binding factor (3)2.50.3
At4g19600Putative protein cyclin C2.50.2
At5g15450HSP100/ClpB2.50.2
At1g11840Lactoylglutathione lyase-like protein2.50.0
At5g42800Dihydroflavonol 4-reductase2.50.1
At1g22370UDP-glucose glucosyltransferase2.40.2
At5g63370Protein kinase2.40.1
At1g36730Eukaryotic translation initiation factor 52.40.1
At3g08860Beta-Ala-pyruvate aminotransferase2.40.2
At5g01600Ferritin 1 precursor2.40.0
At3g52850Vacuolar sorting receptor homolog/AtELP12.40.1
At4g17030Allergen-like protein2.40.1
At5g05110Cys proteinase inhibitor-like protein2.40.1
At3g25230Rotamase FKBP (ROF1)2.30.3
At3g23920Beta-amylase2.30.1
At2g29630Putative thiamin biosynthesis protein2.30.1
At2g20330Putative WD-40 repeat protein2.30.2
At4g23050Ser/Thr kinase MAP3K2.30.4
At4g39210Glucose-1-phosphate adenylyltransferase2.30.2
At2g29300Putative tropinone reductase2.30.3
At5g25390AP2 domain containing protein2.30.1
At2g47470Putative protein disulfide-isomerase2.20.2
At3g59820Leu zipper-EF-hand protein2.20.2
At5g60360Cys proteinase2.20.1
At4g30470Cinnamoyl-CoA reductase2.20.2
At4g35300Putative sugar transporter protein2.20.2
At3g19100CDPK-related kinase2.20.2
At5g65260Poly(A)-binding protein II-like2.20.4
At5g23050Acetyl-CoA synthetase-like protein2.20.3
At5g07920Diacylglycerol kinase (ATDGK1)2.20.2
At4g04020Putative fibrillin2.20.1
At3g20500Purple acid phosphatase2.10.1
At1g67360Stress-related protein2.10.1
At5g53120Spermidine synthase2.10.1
At4g19230Cytochrome P4502.10.1
At1g64550ABC transporter protein2.10.2
At1g62710Beta-VPE2.10.1
At5g24800Transcription factor light-induced CPRF-22.10.3
At1g48000myb-related transcription factor2.10.6
At1g73680Feebly-like protein2.10.2
At5g48030DnaJ protein-like2.10.2
At1g30500Transcription factor2.00.2
At5g62190RNA helicase2.00.1
At1g56170Transcription factor2.00.0
At1g79560FtsH protease, chloroplast2.00.1
At3g06400Putative ATPase (ISW2-like)2.00.2
At3g1127026S proteasome regulatory subunit S122.00.1
At1g64140Similar to putative disease resistance protein2.00.1
At5g10930Ser/Thr protein kinase SNFL32.00.1
At5g51070ATP-dependent Clp protease (ClpD)2.00.1
At5g02620Ankyrin-like protein2.00.4
At3g19170Metalloprotease 12.00.1
At5g53400Similar to nuclear movement protein nudC2.00.1
At4g23670Putative major latex protein 12.00.1
At4g30490Putative ATPase2.00.4
At5g17220Glutathione S-transferase2.00.1
At4g35790Putative protein phospholipase D2.00.3
Section D
At1g52560Chloroplast-small heat shock protein6.70.8
At1g71000Heat shock protein DnaJ4.81.4
At5g59330Nonspecific lipid-transfer protein4.70.9
At2g26150Putative heat shock transcription factor4.01.7
At4g28350Lectin receptor-like Ser/Thr kinase3.70.8
At3g11020DREB2B transcription factor3.61.8
At1g32560Late-embryogenesis abundant protein3.41.7
At1g04220Putative beta-ketoacyl-CoA synthase3.30.5
At2g34355Nodulin-like protein3.10.6
At4g25000Alpha-amylase3.10.3
At5g15250FtsH protease2.90.9
At2g42270Putative ATP-dependent RNA helicase2.90.3
At1g17665Dehydrogenase-like protein2.61.2
At1g02300Cathepsin B-like Cys protease2.60.1
At3g03470Putative cytochrome P4502.60.2
At5g66110atfp6-like protein2.50.5
At4g02430Similar to alternative splicing factor ASF2.50.4
At1g14040Polytropic retrovirus receptor2.50.7
At5g49990Permease2.40.1
At5g43920WD-repeat protein-like2.40.6
At2g40350AP2 domain transcription factor2.30.5
At2g14120Dynamin-like protein2.30.7
At3g50980Dehydrin-like protein dehydrin Xero22.30.4
At1g78670Gamma glutamyl hydrolase2.30.0
At1g47960Similar to invertase inhibitor2.30.1
At2g43570Endochitinase isolog2.30.1
At1g21460Similar to nodule development protein2.20.1
At4g01120GBF2, G-box binding factor2.20.1
At1g07720Fatty acid elongase 3-ketoacyl-CoA synthase2.20.0
At2g15880Related to disease resistance Pro-rich2.20.8
At1g15550Gibberellin 3 beta-hydroxylase2.20.2
At1g77510Putative thioredoxin2.20.1
At4g28450SOF1-like protein (rRNA processing)2.20.1
At4g33490Nucellin-like protein2.20.2
At3g62090Putative phytochrome-associated protein 32.11.0
At3g12860Putative nucleolar protein2.11.2
At1g61580Ribosomal protein2.10.1
At1g04980Disulfide isomerase-related protein2.10.3
At5g58770Dehydrodolichyl diphosphate2.10.0
At1g10050Putative xylan endohydrolase2.10.7
At5g54080Homogentisate 1,2-dioxygenase2.10.2
At2g32920Putative protein disulfide isomerase2.10.1
At2g38530Putative nonspecific lipid-transfer protein2.10.2
At1g80110Similar to PP2 lectin polypeptide2.10.2
At4g24280Heat shock 70 protein2.10.1
At4g30960Ser/Thr protein kinase2.10.1
At3g54820Aquaporin MIP-like protein2.00.1
At2g07750Putative RNA helicase2.01.4
At3g10410Putative Ser carboxypeptidase2.00.2
At5g65110Acyl-CoA oxidase2.00.1
At3g23990Mitochondrial chaperonin hsp602.00.1
At5g24870RING finger-like protein RING-H22.00.5
At3g62560Small GTP-binding protein2.00.1
At3g13784Beta-fructofuranosidase2.00.2
At3g44880Lethal leaf-spot 1 homolog Lls12.00.1
At5g57900SKP1 interacting partner 12.00.1
At5g04810Salt-inducible protein2.00.1
  • Changes in steady-state transcript abundance in plants subjected to a combination of drought and heat stress. Results are presented as fold-difference in steady-state transcript level (log2) over control unstressed plants. Only transcripts with a known (or putative) function and an expression level of fourfold or higher (2 log2) are shown. Accession numbers are given to each transcript on left. The known or putative function of each transcript is given on right. RNA preparation and analysis by Affymetrix chips are described in “Materials and Methods.” Transcripts elevated during a combination of drought and heat stress, as well as drought or heat stress, are indicated in A; transcripts elevated during a combination of drought and heat stress, as well as heat stress, are indicated in B; transcripts elevated during a combination of drought and heat stress, as well as drought, are indicated in C; and transcripts specifically elevated during a combination of drought and heat stress are indicated in D. sd, Standard deviation.