Table II.

Metabolites detected by GC-MS in polar extracts of Arabidopsis leaves subjected to heat stress, drought, or a combination of drought and heat stress

CHDH+D
DerivativeResp ratioaseb%CResp ratiose%CResp ratiose%CResp ratiose
Citric acid0.010.001.100.010.003.620.020.010.690.000.00
Fumaric acid2.710.680.671.820.180.752.041.231.694.580.12
Furanone0.010.000.820.010.001.370.020.000.970.010.00
Glucoheptonic acid meox1 tms0.020.011.460.030.011.120.030.000.800.020.01
Glucoheptonic acid meox2 tms0.010.001.310.010.001.540.020.001.300.010.01
Hydroxybutanoic acid0.040.031.080.040.030.330.010.000.930.040.01
Hydroxysuccinic acid0.220.052.400.530.300.730.160.017.461.640.09
Isocitric acid0.040.031.080.040.030.330.010.000.930.040.01
Lactic acid0.000.002.120.010.011.190.010.000.760.000.00
Malic acid0.010.000.820.000.00NDNDND0.710.000.00
Succinic acid0.060.020.690.040.010.220.010.010.560.040.00
4-Aminobutyric acid0.000.000.840.000.00NDNDND0.620.000.00
Allothreonine0.020.010.610.010.000.250.000.000.630.010.00
Arg0.020.010.190.000.00NDNDND0.290.000.00
β-Ala0.020.011.700.030.013.350.070.030.570.010.00
Cys0.000.001.150.000.000.640.000.003.300.010.00
Glu0.141.370.330.050.030.130.020.000.630.090.02
Gln0.010.011.750.030.011.090.020.024.920.070.02
Gly0.020.010.880.020.010.160.000.001.010.020.01
His0.000.000.910.000.000.650.000.000.620.000.00
Isoleucine0.020.010.190.000.00NDNDND0.290.000.00
Leu0.010.000.440.010.001.370.020.000.970.010.00
Lys0.020.011.460.030.011.120.030.000.800.020.01
Orn0.000.000.690.000.001.000.000.001.250.000.00
Pro0.000.001.280.010.0031.480.130.080.200.000.00
Thr0.050.040.630.030.020.220.010.001.610.080.02
Tyr0.000.000.860.000.002.700.010.000.740.000.00
Val0.020.011.700.030.013.350.070.030.570.010.00
1,3-Diaminopropane0.010.001.100.010.003.620.020.010.690.000.00
Ethanolamine0.040.010.580.020.013.140.120.022.960.110.02
Putrescine0.350.070.720.260.093.151.110.081.550.550.09
Fructose-6-phosphate0.180.010.820.150.032.870.520.052.520.460.09
Glycerol0.120.031.350.160.050.720.080.000.310.040.02
Inositol0.300.131.060.310.071.520.450.062.570.760.14
Lactitol0.100.053.490.340.070.480.050.000.560.050.02
Maltitol0.060.020.850.050.020.570.030.011.090.060.01
Mannitol0.070.040.560.040.030.390.030.001.710.120.01
Xylitol0.010.001.020.010.003.590.040.012.330.030.01
Fructose meox1 tms0.350.071.010.360.053.151.110.081.550.550.09
Fructose meox2 tms0.180.010.900.160.012.870.520.052.520.460.09
Fucose0.220.052.400.530.300.730.160.017.461.640.09
Galactose0.410.041.110.450.024.721.930.034.311.770.30
Glucose0.290.031.520.440.134.561.320.063.921.130.20
Gulose0.050.031.560.080.030.280.010.014.850.240.04
Isomaltose meox1 tms0.060.020.850.050.020.570.030.011.090.060.01
Isomaltose meox2 tms0.010.000.210.000.001.200.020.003.560.050.01
Maltose0.010.011.680.020.010.820.010.005.370.080.02
Mannose1.970.131.132.230.104.378.620.513.907.680.89
Melibiose0.170.072.380.400.160.460.080.034.660.780.17
Sucrose meox1 tms0.430.391.500.650.075.482.371.0423.6510.220.81
Sucrose meox2 tms0.010.010.540.000.000.600.000.0016.250.110.03
Trehalose0.290.031.520.440.134.561.320.063.921.130.20
  • GC-MS analysis of polar extracts from plants subjected to heat stress, drought, or a combination of heat stress and drought. Polar extracts were derivatized and analyzed as described in “Materials and Methods.” All samples were methoximated and trimethylsilylated. Compounds with a twofold increase or more are indicated in bold.

  • a Response ratios are peak areas compared to the internal standard ribitol/adonitol. Peak areas were integrated with Genesis algorithm in Xcalibur.

  • b n = 3. se, Standard error; ND, not detected. Values of 0.00 are <0.01.