Table I.

Purification of the chimeric MBP-Cah6

StepTotal ActivityProteinSpecific ActivityRecovery 100%Purification Fold
WAUamgbWAU/mg
Sonicated cells1,2008,0000.151001
Amylose column8003300672,000
Centricon column4001400332,567
  • a One WAU = (t0−t)/t where t0 is the time for uncatalyzed reaction and t is the time for the enzyme catalyzed reaction.

  • b Determined by Lowry's protein assay (Lowry et al., 1951).