Table II.

Intracellular localization of Cah6 using wild type C. reinhardtii cells

LocationAreaImmunogold Density
ImmunePreimmuneDifference
μm2No. of Immunogold Particles/μm2
Outside7.80 ± 0.381.67 ± 0.101.54 ± 0.110.13 ± 0.19
Cytoplasm6.84 ± 1.20.44 ± 0.020.30 ± 0.020.14 ± 0.03
Nucleus0.36 ± 0.050.47 ± 0.020.47 ± 0.020.00 ± 0.01
Stroma5.93 ± 1.073.0 ± 0.140.85 ± 0.042.15 ± 0.13
Pyrenoid1.85 ± 0.300.99 ± 0.080.97 ± 0.70.02 ± 0.03
Starch sheath0.86 ± 0.1110.9 ± 1.92.3 ± 0.148.6 ± 1.95
  • Immunogold densities in different cell compartments in sections were calculated by dividing the number of immunogold particles in a particular cell organelle with the area of that cell organelle. The data presented in the table is the average ± sd of 15 cell sections. The stroma includes the thylakoid area. The cytoplasmic area was calculated by subtracting the total area of chloroplast and the nucleus from the cell area. The Cah6 antibody dilution used for immunolocalization is 1:10. The cells used for immunolocalization are air acclimated D66 cells grown in minimal medium.