Table II.

Interaction of RGLs and RGA with SLY1 and sly1-d in yeast two-hybrid assays

LexA DB FusionGal4 AD FusionHis Media + 3-ATaβ-gal Unitsb
mm
SLY1RGL121.5 ± 0.2
SLY1RGL200.1 ± 0.1
SLY1RGL351.4 ± 0.2
SLY1RGA20.7 ± 0.1
sly1-dRGL157.1 ± 1.3
sly1-dRGL230 43.0 ± 5.9
sly1-dRGL330 34.0 ± 1.5
sly1-dRGA60 118.7 ± 16.1
LexARGL111.0 ± 0.0
LexARGL200.1 ± 0.1
LexARGL300.1 ± 0.0
LexARGA00.1 ± 0.1
SLY1Gal4cNdd
sly1-dGal4Nd
  • a The relative growth on His plates containing 3-aminotriazole (3-AT) at 0, 1, 2, 5, 10, 30, and 60 mm. 3-AT is a competitive inhibitor of the His3 enzyme. The experiment was repeated twice.

  • b β-gal activity (units). For each sample, at least three independent enzyme assays were performed and the means ± se are shown.

  • c No growth on His plates at 0 mm 3-AT.

  • d Nd, Not determined.