Table II. Transcriptomic and protein phosphorylation data for selected NF-induced genes involved in cell dynamics

The eight right columns indicate detected NF-induced phosphorylation (P) sites and the regulation levels (fold change) following NF treatment for the indicated times or S. meliloti inoculation at 5 d post inoculation (RH-5dpi; skl mutant background) determined in this study versus previous studies: a, Rose et al. (2012); b, van Zeijl et al. (2015); c, Czaja et al. (2012); and d, Breakspear et al. (2014). For entries in parentheses, differences were not statistically significant (adjusted P > 0.05). ART, All root tissues from whole root systems (a) or 1- to 2-cm-long root segments (b and c); ND, not detected; NF, RNAseq reads detected only in the NF-treated samples.

Mt20120830MtV4.0Functional CategoryAnnotationP Sites (a)LCM-4hLCM-24hART-3h (b)ART-6h (c)ART-24h (c)RH-24h (d)RH-5 dpi (d)
Mt0017_10221Medtr3g010330Cell wallCellulase3-like15.319.61.41.933.3
Mt0006_00323Medtr3g082440Cell wallKOR2 endoglucanase10.11.83.35.56.14.0
Mt0008_01038Medtr0118s0070Cell wallExpansin EXPB14-like740.3152.5177.1259.6118.493.5795.4
Mt0004_11139Medtr1g073320Cell wallPlasmodesmata-located protein3-like20.90.2
Mt0015_10081Medtr8g006790TransportPlasma membrane proton pump MtAHA1NFNF268.811.6149.6
Mt0009_00137Medtr8g087710TransportMtNip1 (NOD26)384.0187.2206.38.15.156.0688.2
Mt0005_10852Medtr5g023240TransportLys-His transporterS2749.231.63.410.85.735.6164.2
Mt0040_00155Medtr3g112460TransportPeptide transporter PTR1-like163.1121.3433.93.35.6
Mt0023_10223Medtr2g011570TransportEquilibrative nucleotide transporterNFNF
Mt0007_00517Medtr3g098930TransportSugar transporter SWEET13NFND652.523.2
Mt0006_10110Medtr3g087730TransportSulfate transporter1,335.9337.0105.4
Mt0009_00487Medtr8g095360Membrane traffickingPatellin7.9366.44.32.61.95.131.3
Mt0037_10126Medtr8g038220Membrane traffickingMtAnn2 annexin447.60.2149.5
Mt0057_00074Medtr1g105930Membrane traffickingInositol-1,3,4-trisphosphate5/6-kinase6.810.24.82.42.63.626.0
Mt0036_00256Medtr7g116710CytoskeletonABIL (ABI-like)S2553.966.14.51.41.720.3
Mt0006_10532Medtr3g078623CytoskeletonActin-binding formin-like protein8579.1(NF)755.6
Mt0013_00466Medtr3g060900CytoskeletonKinesin-4 microtubule binding136.11.34.320.2
Mt0036_00045Medtr7g112420CytoskeletonKinesin-4 microtubule bindingS2774.76.23.12.2
Mt0054_10146Medtr6g061690CytoskeletonMicrotubule-associated protein MAP65NF(22.1)1,699.6
Mt0001_10069Medtr4g133890CytoskeletonMicrotubule-associated protein MAP7010.737.711.24.73.48.015.0
Mt0031_00057Medtr7g099290CytoskeletonMYOSIN2S11172.010.9
Mt0040_10068Medtr3g110720CytoskeletonTubulin α-4 chainS4397.12.7
Mt0001_10832Medtr4g116870Cell cycleDNA replication licensing factor316.12.68.1