Table III. Transcriptomic data for selected NF-induced genes involved in perception, signaling, and gene expression

The seven right columns indicate the regulation levels (fold change) following NF treatment for the indicated times or S. meliloti inoculation at 5 d post inoculation (RH-5dpi; skl mutant background) determined in this study versus previous studies: a, van Zeijl et al. (2015); b, Czaja et al. (2012); and c, Breakspear et al. (2014). ART, All root tissues from 1- to 2-cm-long root segments; ND = not detected; NF = RNAseq reads detected only in the NF-treated samples.

Mt20120830MtV4.0Functional CategoryAnnotationLCM-4hLCM-24hART-3h (a)ART-6h (b)ART-24h (b)RH-24h (c)RH-5 dpi (c)
Mt0012_10650Medtr5g033490Receptor-like (RD) kinaseMtLYK10/EPR3NFNF10.88.77.813.950.8
Mt0057_10185Medtr1g104890Receptor-like (RD) kinaseCys-rich RLK160.83.68.01.32.7
Mt0006_00443Receptor-like (non-RD) kinaseMtSymCRK101.7NF2.81.68.340.0
Mt0039_00125Medtr1g088930Receptor-like (non-RD) kinaseLRR RLK51.5NF10.6
Mt0077_10096Medtr2g068650Receptor-like (non-RD) kinaseG-type lectin S RLK66.715.819.1
Mt0036_00102Medtr7g113490Protein kinaseMAPKKK1 ANP1-like16.63.52.7
Mt0068_00014Medtr2g023890Protein kinaseMAPKKK15-like17.819.84.26.93.412.0
Mt0016_00257Medtr2g100290Protein kinaseProtein kinase9.0NF31.81.81.6
Mt0020_00194Medtr8g074920Protein kinaseMtSPK2 protein kinase2NF988.0236.638.620.820.2118.7
Mt0015_10342Medtr8g012795DefenseDefensin-like protein1,025.5559.31,292.021.563.746.725.5
Mt0003_11544Medtr4g085800Immunity controlMtDNF287.9NF4.1
Mt0101_10077Medtr2g437800SignalingCLE-related peptide55.6ND
Mt0020_10418SignalingMtCEP7 peptide162.013.6
Mt0004_01114Medtr1g075730SignalingProtein phosphatase2C27.87.915.3
Mt0026_00412Medtr7g075900SignalingSubtilase, AtSBT1.1 orthologNFNF
Mt0003_00135Medtr4g053630SignalingSubtilase, LjSBTS/AtAIR3-like3.613.77.417.84.83.225.6
Mt0001_11381Medtr4g102400SignalingSubtilase, P69C-like39.41,373.417.175.941.136.753.4
Mt0036_10297Medtr7g117415ProteasomeE3 ubiquitin-protein ligase7.61.84.71.51.75.6
Mt0008_00403Medtr5g061290ProteasomeSeven-in-absentia (SINA) proteinNFNF134.62.83.472.6
Mt0011_00804Medtr7g078150ProteasomeZinc finger, RING/FYVE/PHD-type136.577.74.32.52.110.233.6
Mt0009_00840Medtr8g103227ProteasomeMtLIN-like E3 ligaseNFNF21.0
Mt0042_00049Medtr7g106340ProteasomePlant U-box22 (PUB22)1.63.62.82.32.1
Mt0012_00744Medtr5g031880Transcriptional regulationMtPLT3 ethylene response factorNFNF13.21.11.7
Mt0004_10921Medtr1g069725Transcriptional regulationGRAS SCARECROW-like TF40.7NF11.82.47.5
Mt0043_10032Medtr7g096530Transcriptional regulationLateral organ boundaries domain TF53.6285.34.6
Mt0010_00697Medtr5g090970Transcriptional regulationNo apical meristem TF (ANAC048-like)9.53.43.52.2