Table III.

Comparison of expression changes for selected salt cress transcripts

AGIFunctionLengthLog (C*S)Log (S:C)S:C MAS:C PCR
At1g01720ATAF1773.7270.6324.284.72
At3g04120GapC685.3530.5423.482.43
At1g11910Aspartic proteinase944.4260.0201.051.22
At2g41430ERD15594.634−0.5520.280.24
At5g66190Ferredoxin-NADP+ reductase953.642−0.1180.760.65
At4g26080aABI1a954.115−0.2590.55a2.88a
At4g11650Osmotin704.503−0.4380.370.40
At3g44880Lls1943.8870.1541.430.99
At3g20410CPK9923.801−0.4200.380.52
At2g38540LTP1815.3410.4793.011.41
  • Compared are selected transcripts for which salt cress clones have been obtained by designing salt cress-specific primers and comparing their salt stress to control (S:C) ratio of expression changes determined by real-time RT-PCR (S:C-PCR) to those determined by microarray hybridization to the Arabidopsis long-oligo microarray platform (S:C-MA). Shown are the log10 ratios for intensity (C*S), and log10 (S:C) ratio from microarray experiments, and the fold-change in microarray (MA) and quantitative real-time RT-PCR (PCR) analysis.

  • a The microarray signal was obtained by hybridization to the printed Arabidopsis ABI1-specific oligonucleotide; the real-time PCR signal was obtained by using a primer pair for the cloned ABI1 homolog from salt cress (BQ079252). Several salt cress ESTs (gi|19684239; gi|19913611; gi|19855150; BQ07252), annotated as ABI1 homologs, seem to indicate the existence of ABI1 paralogs in salt cress that may be regulated in a different manner.