Table I. Kinetic parameters of L/ODC and its mutant proteins

All experiments were performed in 50 mm potassium phosphate buffer (at optimal pH for each enzyme). Kinetic parameters were calculated from mean values (n = 3–4). ND, Not detected.

ProteinKmVmaxkcatkcat/KmLDC/ODC Ratio of kcat/Km
LDCODCLDCODCLDCODCLDCODC
mmnmol min−1μg−1s−1m−1 s−1
LcL/ODC-wild type1.695.483.652.463.172.131,8783884.84
LcL/ODC-Y344H22.398.210.512.470.442.14202610.08
NtODC3-wild typeND1.44ND30.30ND23.54ND16,351ND
NtODC3-H344YND0.75ND14.33ND11.33ND14,794ND
NtODC3-H344FND0.61ND3.42ND2.66ND4,368ND