Table I.

List of genes spotted on the mini-array membrane

ColRowGene ProductNo.AnnotationAGI CodeComment
Tetrapyrrole Biosynthetic Genes
11Glutamyl-tRNA reductase3HEMA1At1g58290
12HEMA2At1g09940
13HEMA3At2g31250By homology
14Glu 1-semialdehyde aminotransferase2GSA1At5g63570By homology
21GSA2At3g48730By homology
225-aminolevulinic acid dehydratase2ALAD1At1g69740By homology
23ALAD2At1g44318By homology
24Porphobilinogen diaminase1PBGDAt5g08280
31Uroporphyrinogen III synthase1UROSBy homology, GenBank; AC002504
32Uroporphyrinogen III decarboxylase2URO1At2g40490By homology
33URO2At3g14930By homology
34SAM uroporphyrinogen III methyltransferase1UPM1At5g40850Complemented yeast UPM mutant
41Coproporphyrinogen III oxidase2CPO1At1g03480Mutant isolated as lin2
42CPO2GenBank; AC005275, Pseudogene?
43CPO3At5g63290Bacterial O2-independent type
44Protoporphyrinogen IX oxidase2PPO1At5g14220By homology
51PPO2At4g01690
52Ferrochelatase2FC1At5g26030
53FC2At2g30390
54Heme oxygenase4HO1At2g26670Mutant isolated as hy1
61HO2At2g26550
62HO3At1g69720By homology
63HO4At1g58300By homology
64Phytochromobilin synthase1HY2At3g09150Mutant isolated as hy2
71Mg-chelatase ChlI2CHLI1At4g18490
72CHLI2At5g45930
73ChlH1CHLHAt5g13630Mutant isolated as gun5
74ChlD1CHLDAt1g08520
81SAM Mg-protoporphyrin IX methyltransferase1CHLMAt4g25080
82Mg-protoporphyrin IX monomethylester cyclase1CRD1At3g56940
83Protochlorophyllide oxidoreductase3PORAAt5g54190
84PORBAt4g27440
91PORCAt1g03630
92Chlorophyll synthetase1CHLGAt3g51820
93Chlorophyll a oxygenase1CAOAt1g44446
Control Genes
94Monogalactosyl diacylglycerol synthetase3MGD1At4g31780Chloroplast marker
101MGD2At5g20410Chloroplast marker
102MGD3At2g11810Chloroplast marker
103Response regulator1ARR5At3g48100Cytokinin-responsive
104Low temperature and salt responsive protein1RCI2aAt3g05880Abscisic acid responsive
11112-Oxophytodienoate reductase2OPR1At1g76680Jasmonate-responsive
112OPR2At1g76690Jasmonate-responsive
113Aconitate hydratase1ACOAt4g35830Ethylene-responsive
114Pathogenesis related protein 1 (PR1)1PR1At2g14610Salicylate-responsive
121Actin (housekeeping)1ACT8At1g49240House keeping
122Polyubiquitin (UBQ10)1UBQ10At4g05320House keeping
123Light-harvesting Chl a/b binding protein1Lhcb6At1g15820Chloroplast marker
124Negative control (λDNA)1Negative control
  • A total of 35 genes involved in tetrapyrrole biosynthesis found in the database of the complete genome sequence of Arabidopsis were spotted together with control genes. It should be noted that the annotation of isoforms may vary from those in previous publications.