Table I. List of fragment ions generated in the mass spectrum and tandem mass spectrum related to the peptide identification from the tryptic digests of ReCHS1 and ReCHS2 proteins
ProteinRetention TimePrecursor IonCharge (Ionization Mode)Theoretical MassExperimental MassErrorSequence LocationPeptide Sequence
minm/zm/zppm
ReCHS112.42630.7983 [M+2H]+22 (+)1,259.58221,259.58160.48A44–A54VTNSDHMTDLK
15.46580.2929 [M+2H]+22 (+)1,158.57081,158.57030.4A96–A105QDMVVSEVPR
16.33828.4812 [M+H]+11 (+)827.4737827.4753−1.94A271–A278DVPGLISK
17.38847.4048 [M+3H]+33 (+)2,537.18352,537.1964−5.08A125–A147ITHVIMCTTSGVDMPGADYQLTK
1,270.600 [M+2H]+2
18.24421.5535 [M+3H]+33,2 (+)1,261.63661,261.6377−0.8A70–A79YMHLTEDLLK
631.8258 [M+2H]+2
ReCHS25.61553.2109 [M+H]+11,1 (+)552.2030552.2036−1.09A60–A63MCDK
575.1909 [M+Na]+1
13.12430.3021 [M+H]+11 (+)429.2944429.2951−1.56A318–A321LGLK
13.51607.309 [M+H]+11 (+)606.3044606.3047−1.94A64–A68SMIEK
15.46491.8245 [M+2H]+22,1 (+)981.6338981.63350.31A148–A156LLGLRPSVK
982.6379 [M+H]+1
18.55482.2695 [M+2H]+22,1 (+)962.5235962.51855.13A262–A270LGSPFISSR
963.5290 [M+H]+1
18.96933.9299 [M+2H]+22 (+)1,864.84131,864.8423−0.5A158–A173FMMYQQGCFAGGTVLR