Table III.

Rate constants and half-times for FRAP

LabelCell Typekt1/2
s−1s
YFPHypocotyl0.16 ± 0.036.3 ± 0.8
FDAHypocotyl0.15 ± 0.0215.9 ± 1.1
GFP-mTalinHypocotyl0.05 ± 0.00513.4 ± 1.2
GFP-fABD2Hypocotyl0.05 ± 0.00613.4 ± 1.0
Root hair0.05 ± 0.00716.1 ± 3.0
Guard cell0.05 ± 0.00314.7 ± 0.9
Leaf epidermal0.06 ± 0.00813.2 ± 1.3
  • Arabidopsis cells expressing GFP-fABD2, GFP-mTalin, or YFP, or nonexpressing cells labeled with FDA were photobleached, the recovery plotted, and an exponential function fitted. The k and t1/2 were derived from the exponential function. Values are means ± se, n ≥ 12.