Table II. Steady-state kinetic parameters of purified ReCHSs with different starter units

Results are means (n = 3) with sd values below 10% in all the cases.

Starter CoAReCHS1ReCHS2
KmVmaxEfficiency (Vmax/Km)KmVmaxEfficiency (Vmax/Km)
μmpmol min−1 mg−1μmpmol min−1 mg−1
p-Coumaroyl-CoA38.43421.092155.837.00.237
Acetyl-CoA46.63.230.06937.363.51.702
Butyryl-CoA13.91.00.0718.0111.01.373
Hexanoyl-CoA91.651.270.01311.44.70.412
Octanoyl CoA22.01.10.05026.621.40.804