Table I.

Fatty acid composition in leaves of wild-type Arabidopsis, fad5 mutants, fad5 mutants expressing either genomic DNA containing ADS3 or ADS cDNA constructs, and SALK_142406, a T-DNA insertion mutant of ADS3.2

Fatty AcidWild Typefad5Genomic ADS3cDNASALK_142406
Line C1Line C10Line C T3ADS31-71-ADS1ADS31-71-ADS2ADS3
16:014.1 ± 2.219.6 ± 1.815.2 ± 2.014.2 ± 1.514.6 ± 1.816.6 ± 2.117.0 ± 2.116.4 ± 2.413.8 ± 2.0
16:12.7 ± 0.53.1 ± 0.82.9 ± 0.53.1 ± 0.62.6 ± 0.52.1 ± 0.32.0 ± 1.02.8 ± 1.32.9 ± 0.5
16:312.1 ± 1.6n.d.11.2 ± 1.110.0 ± 1.212.4 ± 1.14.5 ± 1.23.5 ± 0.96.6 ± 2.312.3 ± 1.7
18:01.5 ± 0.22.0 ± 0.42.8 ± 0.33.1 ± 0.41.8 ± 0.66.3 ± 0.94.1 ± 0.53.3 ± 1.01.8 ± 0.5
18:15.6 ± 0.26.2 ± 0.45.9 ± 0.44.9 ± 0.33.7 ± 0.76.6 ± 0.25.5 ± 1.25.8 ± 0.94.9 ± 0.6
18:217.0 ± 2.020.3 ± 1.617.5 ± 1.316.3 ± 2.115.8 ± 1.515.2 ± 2.217.3 ± 3.517.7 ± 2.916.2 ± 2.5
18:346.4 ± 3.449.2 ± 2.643.2 ± 2.147.8 ± 2.548.1 ± 2.647.3 ± 2.548.6 ± 2.746.8 ± 2.247.6 ± 3.6
  • Data as mol% of total fatty acids ± sd; n.d., not detectable; C1, C10, and C T3, transgenic fad5 lines expressing genomic ADS3; SALK_142406, ADS3.2 T-DNA insertion mutant.