Table III.

Quantification of GDH protein in different tissue sections of tobacco plants

Number of Gold Particles/μm2
MitochondriaCytosolVacuole
NL PlantsNH4+ PlantsNL PlantsNH4+ PlantsNL PlantsNH4+ Plants
Leaf mesophyll cells3.83 ± 1.35.1 ± 1.3b0.54 ± 0.10.87 ± 0.26bndcndc
Minor veins CC28 ± 4.142 ± 6.9b4.74 ± 0.812.6 ± 3a7 ± 226 ± 8a
Midribs CC21 ± 3.645 ± 3.6a5.5 ± 0.4513 ± 3.5a20 ± 761 ± 13a
Base stems CC52 ± 7.6104 ± 4.3a5.7 ± 0.720 ± 4.2a14 ± 531 ± 10a
Top stems CC30 ± 4.554 ± 8.2a6.2 ± 19.5 ± 0.34andcndc
  • Immunolocalization of the enzyme was performed using transmission electron microscopy on plants grown on NH4+ and NL plants. Values are the mean ± sd of gold particles counted on 3 to 20 different sections. See footnotes for induction levels of GDH protein.

  • a Significant.

  • b Not significant at 0.05 probability level.

  • c nd, Not detectable.