Table V.

Detection of false negatives

The LIMMA procedure was used to compare consecutive sets of concentrations (0.1 cpc against 0 cpc, 1 cpc against 0.1 cpc, etc.). “−1” and “+1” indicate that the gene is flagged by LIMMA as down-regulated or up-regulated, respectively, whereas “0” is used for genes that do not appear to be differentially expressed. All pair-wise comparisons should theoretically be assigned “−1”.

Spike RNA0 vs 0.10.1 vs 11 vs 1010 vs 100100 vs 10001000 vs 10,000
CATMACATMACATMACATMACATMACATMA
100−1−1−1−1
2000−1−1−1
3−1+1−1−1−1−1
400−1−1−1−1
5−10−1−1−1−1
6+1−1−1−1−1−1
700−1−1−1−1
80+1−1−1−1−1
90+1−1−1−1−1
1000−1−1−1−1
11000−1−1−1
1200−1−1−1−1
14000−1−1−1
AgilentAgilentAgilentAgilentAgilentAgilent
100−1−1−10
200−1−1−1−1
3000−1−1−1
4000−1−1−1
500−1−1−10
7000−1−1−1
8−10−1−1−1−1
90+100−1−1
100−10−1−10
11−10−1−1−1−1
1200−1−1−10
14000−1−1−1