Table III.

Determinations of AphVIII gene copy number

The marker gene copy number was deduced from hybridization bands in Southern blot and from real-time PCR determinations as detailed in “Materials and Methods.” Multicopy control is indicated as 259.89 (2×). ND, Not detected; NA, not analyzed. Text in bold indicates mean values ± sd.

MutantCopy NumberMutantCopy Number
SouthernReal-TimeSouthernReal-Time
7040054.1011.00 ± 0.56
259.891185.3711.52 ± 0.01
259.89 (2×)-2.13 ± 0.28106.1311.29 ± 0.04
5.310.00063 ± 0.0003106.20ND0.0026 ± 0.0007
7.81ND0.00075 ± 0.0005141.9010.97 ± 0.44
13.1010.85 ± 0.08146.4311.27 ± 0.42
13.69ND0.00079 ± 0.0003169.8411.03 ± 0.35
17.2310.85 ± 0.24207.3110.97 ± 0.52
19.32NA0.74 ± 0.004209.811.65 ± 0.31
20.4010.68 ± 0.003209.3411.26 ± 0.46
20.7310.64 ± 0.019209.8211.21 ± 0.42
21.74NA1.56 ± 0.58213.94ND0.00095 ± 0.0002
21.8811.19 ± 0.32219.811.36 ± 1.19
21.9011.3 ± 0.50221.811.24 ± 0.55
22.8210.90 ± 0.39242.4411.14 ± 0.74
23.5910.96 ± 0.30255.9211.53 ± 0.17
25.3810.97 ± 0.28256.1011.34 ± 0.36
26.3510.99 ± 0.65257.3411.38 ± 0.28
42.2810.66 ± 0.01258.75NA1.37 ± 0.47
42.4910.71 ± 0.42258.9011.42 ± 0.27
47.7214.1 ± 1.37259.312.00 ± 0.21