Table I.

Comparison of CO2 assimilation rates and Rubisco carbamylation levels measured at ambient CO2 and high CO2 (1.5 or 2.5 kPa pCO2) of selected primary transformants and wild-type plants

Measurements of CO2 assimilation rate were made with LI-COR gas exchange system at 40 Pa CO2, 1,500 μmol quanta m−2 s−1, and 25°C or in a closed mass spectrometric system at 1.5 kPa pCO2. For measurements of Rubisco after carbamylation leaf samples were collected in the growth cabinet at either 2.5 kPa or 40 Pa pCO2 at an irradiance of 500 μmol quanta m−2 s−1 at 25°C.

Primary TransformantsCO2 Assimilation RateRubisco Carbamylation
μmol m−2 s−1%
At 40 Pa pCO2At 1.5 kPa pCO2At 40 Pa pCO2At 2.5 kPa pCO2
A−0.6315.349.0
Q5.021.6814
SS1.919.614
RR1.713.3416
Aa21.626
C22.64340
PP30.043
TT28.05349
NN20.24753
Wild type31.643.654
Wild type24.143.64144