Table IV.

Macroarray expression ratios of P. vulgaris ESTs identified as abundant in the root nodule library

Expression ratios are derived from hybridization of macroarrays spotted with root nodule ESTs and probed with (32P) cDNA synthesized from nodule (N), root (R), leaf (L), stem (S), and pod (P) RNA.

AnnotationFunctional CategoryExpression Ratioa
N/RN/LN/SN/P
Leghemoglobin 2Nodulation23013537
Acyl-ACP thioesteraseFatty acid metabolism22710230
d-Isomer-specific 2-hydroxyacid dehydrogenaseC metabolism4329937
Nodulin-30Nodulation5808552
TMV resistance protein NDefense4477520
Trehalose-6-P phosphataseC metabolism2277122
Genomic DNA chromosome 3Unknown414689
Early nodulin 55-2Nodulation3306818
Unknown proteinUnknown6326854
Ring finger proteinRegulation2276515
LipoxygenaseDefense2206217
EnolaseC metabolism3226013
Actin-depolymerizing factor 2Cell structure3386038
Embryo-specific proteinPlant development3551318
Pyruvate dehydrogenase E1 componentC metabolism2215240
Protein kinaseRegulation2513420
Elicitor-inducible proteinDefense4414916
Dormancy-associated clone AFD1Regulation2344720
Diaminopimelate decarboxylaseProtein metabolism2214719
Villin 2Plant development8164426
Delta-aminolevulenic acid dehydrataseEnergy metabolism6422436
CarboxylesteraseUnknown3422915
Methylmalonate semialdehyde dehydrogenaseEnergy metabolism2273113
Alcohol dehydrogenase class IIIC metabolism2212130
Hypothetical proteinUnknown6212226
Zinc finger proteinRegulation3172412
Nitrogen fixation-like proteinNutrient assimilation6111424
Adenylsuccinate lyase-like proteinC metabolism7221512
Suc synthaseC metabolism8181410
Ankyrin proteinPlant development291811
No apical meristem proteinPlant development69108
  • a Average of two or three independent membrane hybridizations. The highest expression ratio for each EST is indicated by bold numbers.