Table II.

Differential expression of type I MADS box transcription factors and cell cycle genes in pollen in comparison to vegetative tissues

The first column shows whether a gene is enriched in pollen when compared to the vegetative tissues, leaves, seedlings, and siliques, and the second gives the lower confidence bound of the fold change (average of the comparisons of pollen to the vegetative tissues). The third column depicts whether a gene is selectively expressed in pollen (present detection call only in pollen), followed by the Affymetrix probe set and the TAIR locus (AGI ID) assigned to this probe set in the fourth and fifth columns. The sixth column depicts the gene annotation. In the following columns, the expression value of the gene (weighted average of duplicates) and its detection call (P or A) are given for the respective cell type or tissue. For more details, see Supplemental Table III (transcription and cell cycle). FC, Fold change; AGI, Arabidopsis Genome Initiative; P, Present; A, absent.

EnrichedFCSelectiveProbe SetAGI IDAnnotationPollenCallLeafCallSeedlingCallSiliqueCall
MADS Box Transcription Factors (Type I)a
    X19.8    X266994_atAt2g34440AGL29 (M/Mα)915P33A30A32A
    X4.5    X248602_s_atAt5g49420AGL84 (M/Mα)49P6A5A15A
    X2.6    X245591_atAt4g14530Type I MADS box (M)62P19A17A21A
    X2.0    X263735_s_atAt1g60040AGL49 (MADS-like/Mβ)226P93A73A92A
247767_atAt5g58890AGL82 (MADS-like/Mβ)72P68P66P57P
247631_atAt5g60440AGL62 (M/Mα)41P27P21P30P
MADS Box Transcription Factors (MIKC*)b
    X49.5    X255952_atAt1g22130AGL104 (O/Mδ/MIKC*)1569P24A23A21A
    X13.7    X262179_atAt1g77980AGL66 (O/Mδ/MIKC*)1331P75A62A54A
    X4.5    X266793_atAt2g03060AGL30 (O/Mδ/MIKC*)495P106A90A89A
    X4.1261423_atAt1g18750AGL65 (O/Mδ/MIKC*)1015P255P214P148P
MADS Box Transcription Factors (Type II)c
    X12.1251623_atAt3g57390AGL18 (MIKC)4993P246P318P796P
264872_atAt1g24260AGL9 (MIKC)168P49A90P1318P
251312_atAt3g61120AGL13 (MIKC)100P71A62P50P
    X245819_atAt1g26310AGL10 (MIKC)99P105A93A70A
251672_atAt3g57230AGL16 (MIKC)80P365P231P179P
255014_atAt4g09960AGL11 (MIKC)50P203A166A2681P
247224_atAt5g65080AGL68 (MIKC)39P76P52A47P
Cell Cycle CDK (Cyclin-Dependent Kinases)d
262802_atAt1g20930CDKB2;2500P340A418P443P
256372_atAt1g66750CDKD;2481P402P400P399P
253756_atAt4g28980CDKF;1377P570P599P532P
247334_atAt5g63610CDKE;1372P461P402P606P
266401_s_atAt2g38620CDKB1;2260P158A385P553P
255909_atAt1g18040CDKD;3246P116P183P219P
252337_atAt3g48750CDKA;1111P672P647P553P
Cycline
    X5.4246385_atAt1g77390CYCA1;2599P67A97P85A
    X1.9262752_atAt1g16330CYCB3;1337P123A160P147P
    X1.8246938_atAt5g25380CYCA2;187P30A44P52P
249129_atAt5g43080CYCA3;1332P212P287P248P
261780_atAt1g76310CYCB2;4190P147A291P238P
259290_atAt3g11520CYCB1;3146P99A323P669P
264043_atAt2g22490CYCD2;1142P251P236P158P
245739_atAt1g44110CYCA1;1134P114P578P489P
264697_atAt1g70210CYCD1;188P149P139P111P
246762_atAt5g27620CYCH;166P159P225P195P
247190_atAt5g65420CYCD4;151P30A63P57P
CKS (CDK/Cyclin Interactors and Regulatory Proteins)f
    X2.7264061_atAt2g27970CKS21196P203P804P845P
264070_atAt2g27960CKS1156P295P335P344P
RBR1, WEE1, and E2F-DPg
    X2.0257524_atAt3g01330DEL3320P124A152P143P
256268_atAt3g12280RBR1255P255P344P320P
262106_atAt1g02970WEE1199P142P170P172P
  • a Absent call in pollen: AGL23 (At1g65360), AGL28 (At1g01530), AGL33 (At2g26320), AGL37 (At1g65330), AGL40 (At4g36590), AGL87 (At1g22590), AGL96 (At5g06500).

  • b Absent call in pollen: AGL94 (At1g69540).

  • c Absent call in pollen: AGL15 (At5g13790), ABS/TT16 (At5g23260), AGL31 (At5g65050), AGL5 (At2g42830), AG (At4g18960), AGL8 (At5g60910), AGL24 (At4g24540), AGL2 (At5g15800), AGL17 (At2g22630), FLC (At5g10140), FCL2 (At5g65070), AGL12 (At1g71692), AGL42 (At5g62165), AGL1 (At3g58780), AP3 (At3g54340), PI (At5g20240), AGL3 (At2g03710), AGL20 (At2g45660), AGL4 (At3g02310), AGL22 (At2g22540), AGL19 (At4g22950), AGL27 (At1g77080).

  • d Absent call in pollen: CDKB2;1 (At1g76540), CDKC;1 (At5g10270), CDKC;2 (At5g64960).

  • e Absent call in pollen: CYCA2;2 (At5g11300), CYCA2;3 (At1g15570), CYCA2;4 (At1g80370), CYCA3;2 (At1g47210), CYCA3;4 (At1g47230), CYCB1;1 (At4g37490), CYCB1;4 (At2g26760), CYCB2;1 (At2g17620), CYCB2;2 (At4g35620), CYCD3;1 (At4g34160), CYCD3;2 (At5g67260), CYCD3;3 (At3g50070), CYCD5;1 (At4g37630), CYCD6;1 (At4g03270).

  • f Absent call in pollen: KRP1 (At2g23430), KRP2 (At3g50630), KRP3 (At5g48820), KRP5 (At3g24810).

  • g Absent call in pollen: E2Fa (At2g36010), E2Fb (At5g22220), E2Fc (At1g47870), DPa (At5g02470), DPb (At5g03410), DEL2 (At5g14960).