Table II.

Mutant lines analyzed for heat stress phenotypes

Further information is provided in Supplemental Table I. ACC, 1-Aminocyclopropane-1-carboxylic acid; N/A, not applicable.

Mutant AlleleGeneAtg No.Functional Category
aba1-3Zeaxanthin epoxidaseAt5g67030ABA biosynthesis
aba2-1Short-chain dehydrogenase/reductase (SDR protein) involved in the conversion of xanthoxin to ABA-aldehydeAt1g52340ABA biosynthesis
aba3-1Molybdenum cofactor sulfuraseAt1g16540ABA biosynthesis
ade1-1UnknownUnknownEnhanced ABA production
abi1-1Protein phosphatase 2CAt4g26080ABA insensitive
abi2-1Protein phosphatase 2CAt5g57050ABA insensitive
abi3-1ABA-dependent transcription factorAt3g24650ABA insensitive
NahGTransgenic plant expressing the salicylate hydroxylase gene from Pseudomonas syringaeN/ACannot accumulate salicylic acid
npr1-1Similar to the transcription factor inhibitor IκBAt1g64280Does not acquire systemic resistance to pathogens
etr1-1Ethylene receptorAt1g66340Insensitive to ethylene
ein2-1NRAMP metal transporter familyAt5g03280Insensitive to ethylene
eto1-1Negative regulator of ACC synthase 5At3g51770Higher ethylene levels
eto2-1ACC synthase 5At5g65800Higher ethylene levels
eto3-1ACC synthase 9At3g49700Higher ethylene levels
atrbohARespiratory burst oxidase homolog AAt5g07390Unknown
atrbohBRespiratory burst oxidase homolog BAt1g09090Unknown
atrbohCRespiratory burst oxidase homolog CAt5g51060Unknown
atrbohDRespiratory burst oxidase homolog DAt5g47910Unknown
atrbohERespiratory burst oxidase homolog EAt1g19230Unknown
atrbohFRespiratory burst oxidase homolog FAt1g64060Unknown
atrbohGRespiratory burst oxidase homolog GAt4g25090Unknown
atrbohHRespiratory burst oxidase homolog HAt5g60010Unknown
fad2-1Endoplasmic reticulum Δ12 desaturaseAt3g12120Fatty acid synthesis
fad2-2Endoplasmic reticulum Δ12 desaturaseAt3g12120Fatty acid synthesis
fad3-1Endoplasmic reticulum omega-3 fatty acid desaturaseAt2g29980Fatty acid synthesis
fad4-1Predicted to encode the chloroplastic phosphatidylglycerol desaturaseUnknownFatty acid synthesis
fad5-1Chloroplastic fatty acid desaturase family, responsible for synthesis of 16:1 fatty acidsAt3g15850Fatty acid synthesis
fad6-1Chloroplastic omega-6 fatty acid desaturase responsible for the synthesis of 16:2 and 18:2 fatty acidsAt4g30950Fatty acid synthesis
fad7-1Chloroplastic omega-3 fatty acid desaturaseAt3g11170Fatty acid synthesis
fad7-2Chloroplastic omega-3 fatty acid desaturaseAt3g11170Fatty acid synthesis
fad7-1/fad8-1Double mutant in two chloroplastic omega-3 fatty acid desaturasesAt3g11170Fatty acid synthesis
fab2-1Mutant in acyl-carrier-protein desaturase/stearoyl-ACP desaturase (SSI2)At2g43710Fatty acid synthesis
vtc1-1GDP-Man pyrophosphorylaseAt2g39770Vitamin C deficient
vtc2-1Expressed proteinAt4g26850Vitamin C deficient
npq1-2Violaxanthin deepoxidaseAt1g08550Altered nonphotochemical quenching
cad2-1γ-Glutamylcysteine synthetaseAt4g23100Cadmium sensitive
cat1/3Catalase (both peroxisomal)At1g20630/At1g20620Catalase deficient
acd2-2Expressed proteinAt4g37000Spontaneous lesions in the absence of infection
ubp1-1Ubiquitin-specific protease 1At2g32780Ubiquitin specific protease 1
ubp2-1Ubiquitin-specific protease 2At1g04860Ubiquitin specific protease 2
axr1-3Similar to a ubiquitin-like activating enzymeAt1g05180Auxin resistant
uvh6-1DNA repair helicase, presumed transcription factorAt1g03190UV light sensitive
uvh3-1Excision repair nucleaseAt3g28030UV light sensitive
uvh1-1Excision repair nucleaseAt5g41150UV light sensitive
tsl4Unknown geneUnknownGrows at 17°C but not at 37°C
rns1S-like ribonucleaseAt2g02990Induced by inorganic phosphate (Pi) starvation
hot1-1HSP101At1g74310Hsp100/ClpB family HSP