Table III.

Summary of thermotolerance phenotype of mutants at different developmental stages

Wild-type and mutant plants were assayed for thermotolerance at five different developmental stages. Data shown here are all from acquired thermotolerance assays, except in the germination and high light assays, where plants were heated directly to 45°C as indicated. Plant age corresponds to the number of days of growth prior to heat treatment. The more severe mutant phenotypes are shown in boldface, and moderate phenotypes are in italics. Each experiment was repeated at least three times (at least 30 plants assayed in total), except the 25-d assay, where only five plants were assayed in each experiment. Error represents sds. Following are mutants with wild-type phenotype in hypocotyl assays: ade1, abi3, eto1, eto2, eto3, atrbohA, atrbohC, atrbohE, atrbohF, atrbohG, atrbohH, fad2, fad3, fad4, fad5, fad6, fad7, fad7/8, fab3, acd2, ubp1, ubp2, ubp1/ubp2, cat1/3, rns1, tsl4, axr1, and uvh1. WT, Wild-type.

MutantGermination 45°C, 220 min (% Germinated)2.5-d Hypocotyl Elongation 38°C, 90 min, 45°C, 180 min (% Unheated Growth)4-d Root Growth 38°C, 90 min, 45°C, 180 min (% Unheated Growth)7-d Survival 38°C, 90 min, 45°C, 180 min (% WT Survival)7-d Survival, High Light 45°C, 30 min (% WT Survival)TBARS 7 d 38°C 90 min, 45°C 180 min (% WT TBARS)25-d Survival 38°C, 90 min, 45°C, 180 min (Description)
Col93.4 ± 7.622.7 ± 2.542.7 ± 7100100100Green
hot19.1 ± 7.50 ± 014.3 ± 113.3 ± 58.3 ± 10270 ± 38Bleached
aba187.4 ± 9.124 ± 3.817.5 ± 325.4 ± 416.7 ± 12207 ± 26Bleached
aba292.4 ± 8.325 ± 4.818.3 ± 532.1 ± 316.1 ± 12218 ± 59Bleached
aba382.7 ± 7.326 ± 4.316.0 ± 431.7 ± 217.9 ± 11216 ± 72Bleached
abi179.7 ± 16.320.3 ± 3.311.0 ± 423.1 ± 215.4 ± 11294 ± 60Bleached
abi280.0 ± 16.123.8 ± 3.614.9 ± 522.3 ± 1214.2 ± 10238 ± 13Bleached
NahG85.7 ± 21.417.3 ± 3.723.4 ± 440.0 ± 1416.5 ± 19285 ± 54Bleached
npr187.3 ± 21.420 ± 4.439.6 ± 279.4 ± 1278.9 ± 8147 ± 25Bleached patches
ein271.0 ± 7.919.3 ± 2.928.9 ± 469.2 ± 1521.8 ± 16165 ± 37Bleached patches
etr172.3 ± 16.422.9 ± 2.429.3 ± 374.3 ± 922.1 ± 17141 ± 26Bleached patches
atrbohB93.4 ± 7.818.0 ± 2.922.1 ± 249.7 ± 812.1 ± 12254 ± 46Bleached
atrbohD76.8 ± 7.519.0 ± 2.423.3 ± 348.5 ± 109.7 ± 13219 ± 39Bleached
npq189.1 ± 7.424.5 ± 2.829.9 ± 372.9 ± 434.6 ± 17145 ± 37Bleached
cad286.8 ± 12.525.5 ± 2.435.4 ± 477.9 ± 753.5 ± 17159 ± 21Bleached patches
vtc199.8 ± 16.520.0 ± 2.830.7 ± 176.7 ± 316 ± 14159 ± 19Bleached
vtc295.4 ± 8.921.9 ± 2.526.9 ± 272.8 ± 412.1 ± 10164 ± 43Bleached
uvh611.4 ± 11.70 ± 018.6 ± 217.4 ± 913.3 ± 11216 ± 14Bleached
uvh35.2 ± 8.65.4 ± 2.847.3 ± 5.3110 ± 10112 ± 1598.5 ± 35Green